Detailed information of TA system TA219304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4085652..4086249
• Replicon: chromosome
• Accession: NZ_CP084765
• Organism: Klebsiella variicola subsp. variicola strain F2R9T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: LGN93_RS19915
• Protein ID: WP_012542526.1
• Coordinates: 4085932..4086249 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGN93_RS19910
• Protein ID: WP_012542525.1
• Coordinates: 4085652..4085939 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN93_RS19880 (LGN93_19880)	4081290..4081538	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
LGN93_RS19885 (LGN93_19885)	4081555..4081896	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LGN93_RS19890 (LGN93_19890)	4081927..4083042	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
LGN93_RS19895 (LGN93_19895)	4083222..4083803	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
LGN93_RS19900 (LGN93_19900)	4083803..4084171	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
LGN93_RS19905 (LGN93_19905)	4084291..4084944	+	654	WP_032729541.1	oxygen-insensitive NAD(P)H nitroreductase	-
LGN93_RS19910 (LGN93_19910)	4085652..4085939	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
LGN93_RS19915 (LGN93_19915)	4085932..4086249	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGN93_RS19920 (LGN93_19920)	4086434..4087477	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
LGN93_RS19925 (LGN93_19925)	4088007..4088873	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
LGN93_RS19930 (LGN93_19930)	4088982..4090409	+	1428	WP_040973773.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T219304 WP_012542526.1 NZ_CP084765:c4086249-4085932 [Klebsiella variicola subsp. variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T219304 NZ_CP116249:1714034-1714366 [Enterobacter roggenkampii]
ATGGAAAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGAGTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGTAACTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCGGGGACAAAAACAATGGGCGTGATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGAAACGGCATGCGGCTGGAACGTATACCTGACGCCGTTGTAAATGAGGTGCTTGCCAGGCTGGATGC
GATTCTGAGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT219304 WP_012542525.1 NZ_CP084765:c4085939-4085652 [Klebsiella variicola subsp. variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT219304 NZ_CP116249:1713776-1714033 [Enterobacter roggenkampii]
ATGTATACAACTCGTCTTAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAACT
GTCGACGGACAGCGAAGTAGGCATGACCATCAATAATGGCTGCCTGATTATCGAACCTCAGAAACGGCCTCACTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCATGCTGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCAGTA
GGGAAGGAGCTCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2