Detailed information of TA system TA021925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 5546260..5546792
• Replicon: chromosome
• Accession: NC_008786
• Organism: Verminephrobacter eiseniae EF01-2

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A1WSS7
• Locus tag: VEIS_RS24270
• Protein ID: WP_011812662.1
• Coordinates: 5546260..5546532 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A1WSS8
• Locus tag: VEIS_RS24275
• Protein ID: WP_011812663.1
• Coordinates: 5546532..5546792 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VEIS_RS29455	5544153..5544425	+	273	WP_157048663.1	hypothetical protein	-
VEIS_RS30080	5544403..5545398	+	996	WP_198137937.1	hypothetical protein	-
VEIS_RS24265	5545395..5545760	+	366	WP_157048664.1	hypothetical protein	-
VEIS_RS27480	5545753..5546065	+	313	Protein_5159	XRE family transcriptional regulator	-
VEIS_RS24270	5546260..5546532	-	273	WP_011812662.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
VEIS_RS24275	5546532..5546792	-	261	WP_011812663.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
VEIS_RS24280	5547028..5547252	+	225	WP_011812664.1	DUF2442 domain-containing protein	-
VEIS_RS24285	5547333..5549603	-	2271	WP_041950334.1	xanthine dehydrogenase molybdopterin binding subunit	-
VEIS_RS24290	5549816..5551354	-	1539	WP_011812666.1	xanthine dehydrogenase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-82)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10529.09 Da
• Isoelectric Point: 10.0936

>T21925 WP_011812662.1 NC_008786:c5546532-5546260 [Verminephrobacter eiseniae EF01-2]
MRQPGYSDQFKRDVKRAQKRGKDMGKLKTLLSLLIEGKPLSASYLDHPLKGDWRGFRDAHIEPDWLLIYKIAGDIVRFER
TGRHSDLFDE

Nucleotide

• Length: 273 bp

>T21925 NC_008786:c5546532-5546260 [Verminephrobacter eiseniae EF01-2]
ATGCGCCAGCCCGGCTATTCCGACCAGTTCAAGCGCGATGTAAAGCGGGCGCAAAAACGCGGCAAGGACATGGGCAAGCT
CAAGACCCTGTTGAGCTTGCTCATCGAGGGCAAGCCGCTATCAGCATCCTATCTCGACCACCCACTGAAAGGCGACTGGC
GTGGTTTCCGAGATGCACACATTGAGCCTGATTGGCTGCTGATCTACAAGATTGCCGGGGATATTGTGAGGTTTGAGCGC
ACCGGGCGGCATTCAGACTTGTTTGACGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9346.76 Da
• Isoelectric Point: 8.8285

>AT21925 WP_011812663.1 NC_008786:c5546792-5546532 [Verminephrobacter eiseniae EF01-2]
MAATTFVRARIDEALKNEAAAVLAGMGLTVSDVVRIALTRIAKEKTLPFEMRVPNRQTAQTLAKSERGEDVHHAKDANDL
FGQLGI

Nucleotide

• Length: 261 bp

>AT21925 NC_008786:c5546792-5546532 [Verminephrobacter eiseniae EF01-2]
ATGGCTGCAACGACATTTGTCCGGGCGCGGATTGATGAAGCGCTAAAAAACGAAGCCGCCGCCGTACTGGCCGGCATGGG
CCTTACCGTGTCCGACGTGGTGCGTATCGCACTGACCAGGATTGCGAAGGAAAAGACGCTTCCATTCGAGATGCGTGTTC
CTAACAGGCAGACAGCGCAGACACTGGCCAAGAGTGAGCGGGGTGAGGATGTGCACCATGCCAAAGATGCGAATGACCTG
TTTGGGCAACTTGGCATCTGA

Structures

Toxin

• AlphaFold DB: A1WSS7

Antitoxin

• AlphaFold DB: A1WSS8