Detailed information of TA system TA219235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24692..25350
• Replicon: plasmid pHKU5b
• Accession: NZ_CP084729
• Organism: Acinetobacter baumannii strain HKU5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LHJ61_RS19585
• Protein ID: WP_000312250.1
• Coordinates: 24991..25350 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LHJ61_RS19580
• Protein ID: WP_001096429.1
• Coordinates: 24692..24991 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHJ61_RS19545 (LHJ61_19545)	19858..20487	+	630	WP_000701002.1	hypothetical protein	-
LHJ61_RS19550 (LHJ61_19550)	20527..21036	+	510	WP_001043200.1	hypothetical protein	-
LHJ61_RS19555 (LHJ61_19555)	21118..21630	+	513	WP_000840065.1	hypothetical protein	-
LHJ61_RS19560 (LHJ61_19560)	21686..22015	+	330	WP_000459117.1	hypothetical protein	-
LHJ61_RS19565 (LHJ61_19565)	22052..22780	+	729	WP_001022819.1	hypothetical protein	-
LHJ61_RS19570 (LHJ61_19570)	23016..23570	+	555	WP_000790082.1	hypothetical protein	-
LHJ61_RS19575 (LHJ61_19575)	23620..24156	+	537	WP_226511746.1	hypothetical protein	-
LHJ61_RS19580 (LHJ61_19580)	24692..24991	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
LHJ61_RS19585 (LHJ61_19585)	24991..25350	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LHJ61_RS19590 (LHJ61_19590)	25551..26117	+	567	WP_000710385.1	hypothetical protein	-
LHJ61_RS19595 (LHJ61_19595)	26166..26348	+	183	WP_000373385.1	hypothetical protein	-
LHJ61_RS19600 (LHJ61_19600)	26415..27113	+	699	WP_000873188.1	hypothetical protein	-
LHJ61_RS19605 (LHJ61_19605)	27252..27635	+	384	WP_000654348.1	hypothetical protein	-
LHJ61_RS19610 (LHJ61_19610)	27702..28460	+	759	WP_001053128.1	hypothetical protein	-
LHJ61_RS19615 (LHJ61_19615)	28512..28847	+	336	WP_000653925.1	hypothetical protein	-
LHJ61_RS19620 (LHJ61_19620)	29714..30028	+	315	WP_000708715.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..69524	69524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T219235 WP_000312250.1 NZ_CP084729:c25350-24991 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T219235 NZ_CP116222:4153511-4153765 [Providencia vermicola]
ATGAAAACAATTTCATTTAGTGAGGCTAGAAATAATCTAAAATCGGTATTAGATAAAGTTGTTGATGATGCAGATACAAC
TATCATCACTCCTCGTGACTCAGAGGATGCTGGAGTTATGTCTTTAGAGTATTACAATAGCCTAATGGAAACGATTTATT
TATTACGCTCACCAGCAAATGCCGAACATTTAAATAAATCAATTGCTCAATTTAAAGCCGGAAAAGTAAAGAAAAGAGAG
CTTATAAATGAGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT219235 WP_001096429.1 NZ_CP084729:c24991-24692 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT219235 NZ_CP116222:4153758-4154015 [Providencia vermicola]
ATGAGTAGATTTTTAATATGAACTGACCAAGGATGGGAAGACTATCTATACCGGCAAACCCAAGACAAAAAAATGCTCAA
ACGCATTAATAAGCTCATTGACGAGACTAAAAGAACACCTTTTTATGGGATAGGAAAACCAGAACCACTAAAAGAAAACT
TAGCTGGCTTCTGGTCTAGACGAATAGATGAAACAAATAGGCTCATTTATTCAATAGATGATGAGGGTTTAACTATTATT
GCTTGTCGTTATTATTAA