Detailed information of TA system TA219203

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46332..46585
• Replicon: plasmid p2
• Accession: NZ_CP084707
• Organism: Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K8U55_RS28720
• Protein ID: WP_001312851.1
• Coordinates: 46436..46585 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	46332..46391 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8U55_RS28680 (41714)	41714..42058	-	345	Protein_60	IS6-like element IS26 family transposase	-
K8U55_RS28685 (42110)	42110..42814	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K8U55_RS28690 (42839)	42839..43039	+	201	WP_072354025.1	hypothetical protein	-
K8U55_RS28695 (43059)	43059..43805	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
K8U55_RS28700 (43860)	43860..44420	+	561	WP_000139328.1	fertility inhibition protein FinO	-
K8U55_RS28705 (44552)	44552..44752	+	201	WP_015059022.1	hypothetical protein	-
K8U55_RS28710 (45138)	45138..45737	+	600	WP_032083981.1	PIN domain-containing protein	-
K8U55_RS28715 (45799)	45799..46131	+	333	WP_152916585.1	hypothetical protein	-
- (46332)	46332..46391	-	60	NuclAT_1	-	Antitoxin
- (46332)	46332..46391	-	60	NuclAT_1	-	Antitoxin
- (46332)	46332..46391	-	60	NuclAT_1	-	Antitoxin
- (46332)	46332..46391	-	60	NuclAT_1	-	Antitoxin
K8U55_RS28720 (46436)	46436..46585	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K8U55_RS28725 (46871)	46871..47119	+	249	WP_000083837.1	replication regulatory protein RepA	-
K8U55_RS28730 (47234)	47234..47412	+	179	Protein_70	protein CopA/IncA	-
K8U55_RS28740 (47431)	47431..48288	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
K8U55_RS28745 (49227)	49227..49880	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K8U55_RS28750 (49973)	49973..50230	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K8U55_RS28755 (50163)	50163..50564	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K8U55_RS28760 (50813)	50813..51228	+	416	Protein_75	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..131273	131273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T219203 WP_001312851.1 NZ_CP084707:46436-46585 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T219203 NZ_CP116197:3803105-3803383 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT219203 NZ_CP084707:c46391-46332 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin