Detailed information of TA system TA021920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4530134..4530712
• Replicon: chromosome
• Accession: NC_008786
• Organism: Verminephrobacter eiseniae EF01-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A1WQD0
• Locus tag: VEIS_RS20005
• Protein ID: WP_011811824.1
• Coordinates: 4530452..4530712 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VEIS_RS20000
• Protein ID: WP_011811823.1
• Coordinates: 4530134..4530442 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VEIS_RS19980	4525511..4526272	+	762	WP_011811819.1	IS110 family transposase	-
VEIS_RS19985	4526353..4527087	+	735	WP_011811820.1	GntR family transcriptional regulator	-
VEIS_RS29155	4527336..4527608	+	273	WP_011811821.1	hypothetical protein	-
VEIS_RS19995	4527605..4529902	+	2298	WP_011811822.1	hypothetical protein	-
VEIS_RS20000	4530134..4530442	-	309	WP_011811823.1	HigA family addiction module antidote protein	Antitoxin
VEIS_RS20005	4530452..4530712	-	261	WP_011811824.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VEIS_RS26955	4530654..4530860	-	207	WP_198138085.1	hypothetical protein	-
VEIS_RS29925	4531160..4531492	+	333	WP_011811825.1	hypothetical protein	-
VEIS_RS20015	4531584..4532786	-	1203	WP_011811826.1	cystathionine beta-lyase	-
VEIS_RS29160	4532836..4532988	-	153	WP_157048605.1	hypothetical protein	-
VEIS_RS20020	4532998..4534008	-	1011	WP_011811827.1	tripartite tricarboxylate transporter substrate binding protein	-
VEIS_RS29930	4534084..4534365	-	282	WP_198138086.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (18-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10211.64 Da
• Isoelectric Point: 10.5735

>T21920 WP_011811824.1 NC_008786:c4530712-4530452 [Verminephrobacter eiseniae EF01-2]
VTRHEALFRSQCVPRFKNIERVARRKLLQLHAATEWASLRVPPGNRLEALKGDRRGQHSIRIDDQWRVCFVWHDDGARQV
EIVDYH

Nucleotide

• Length: 261 bp

>T21920 NC_008786:c4530712-4530452 [Verminephrobacter eiseniae EF01-2]
GTGACGCGACACGAGGCGCTTTTCCGCAGCCAGTGTGTGCCCCGGTTCAAGAACATTGAACGTGTGGCACGGCGAAAGCT
CTTGCAACTGCATGCCGCTACTGAATGGGCCAGTTTGCGGGTTCCCCCCGGGAACCGGCTCGAAGCGTTGAAGGGCGACC
GCCGGGGGCAACACAGTATCCGCATCGATGACCAGTGGCGCGTCTGTTTCGTATGGCATGACGACGGTGCCCGCCAGGTC
GAAATCGTGGACTACCACTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11698.55 Da
• Isoelectric Point: 6.3274

>AT21920 WP_011811823.1 NC_008786:c4530442-4530134 [Verminephrobacter eiseniae EF01-2]
MAELLDEIHPGEILLEDFMKPMGISARQLAADIDVSPSRISDLVHGNRPITADTALRLGLFFDMEPRFWMNLQTEYDMRV
ATRTVREKIALRIRVFHHHAAA

Nucleotide

• Length: 309 bp

>AT21920 NC_008786:c4530442-4530134 [Verminephrobacter eiseniae EF01-2]
ATGGCTGAACTACTCGACGAAATCCACCCTGGCGAAATCCTGCTGGAGGACTTCATGAAGCCGATGGGAATATCTGCGCG
CCAGCTTGCGGCAGACATTGACGTCTCACCCAGCCGGATCAGTGATCTGGTGCACGGAAATCGTCCGATCACCGCTGATA
CGGCTTTGCGGCTCGGTTTGTTTTTCGACATGGAGCCGCGATTCTGGATGAATCTGCAAACGGAGTACGACATGCGCGTC
GCGACACGCACCGTGCGCGAAAAAATCGCGCTCAGGATTCGTGTTTTCCATCATCATGCCGCCGCATGA

Structures

Toxin

• AlphaFold DB: A1WQD0