Detailed information of TA system TA219156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1279003..1279733
• Replicon: chromosome
• Accession: NZ_CP084692
• Organism: Enterobacter mori strain BC01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W2Z921
• Locus tag: K6742_RS05960
• Protein ID: WP_032662028.1
• Coordinates: 1279419..1279733 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6742_RS05955
• Protein ID: WP_152537873.1
• Coordinates: 1279003..1279422 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6742_RS05930 (K6742_05930)	1274451..1275956	-	1506	WP_214594889.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
K6742_RS05935 (K6742_05935)	1275953..1276546	-	594	WP_108417255.1	transcriptional regulator UhpA	-
K6742_RS05940 (K6742_05940)	1276672..1276959	-	288	WP_010426528.1	acetolactate synthase small subunit	-
K6742_RS05945 (K6742_05945)	1276963..1278651	-	1689	WP_214594888.1	acetolactate synthase large subunit	-
K6742_RS05950 (K6742_05950)	1278758..1278856	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
K6742_RS05955 (K6742_05955)	1279003..1279422	-	420	WP_152537873.1	helix-turn-helix domain-containing protein	Antitoxin
K6742_RS05960 (K6742_05960)	1279419..1279733	-	315	WP_032662028.1	type II toxin-antitoxin system HigB family toxin	Toxin
K6742_RS05965 (K6742_05965)	1279739..1280116	+	378	WP_214594969.1	hypothetical protein	-
K6742_RS05970 (K6742_05970)	1280220..1280309	+	90	WP_023309830.1	type I toxin-antitoxin system toxin TisB	-
K6742_RS05975 (K6742_05975)	1280374..1280820	+	447	WP_174340318.1	GNAT family N-acetyltransferase	-
K6742_RS05980 (K6742_05980)	1280883..1281716	+	834	WP_214594887.1	EamA family transporter	-
K6742_RS05985 (K6742_05985)	1281892..1283076	+	1185	WP_214594886.1	multidrug efflux MFS transporter EmrD	-
K6742_RS05990 (K6742_05990)	1282979..1284067	-	1089	WP_214594885.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12375.48 Da
• Isoelectric Point: 10.2219

>T219156 WP_032662028.1 NZ_CP084692:c1279733-1279419 [Enterobacter mori]
MHLISMKAILDAVTQFPQHKEELLFLGRTIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T219156 NZ_CP116191:c3606802-3606467 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15473.06 Da
• Isoelectric Point: 4.9501

>AT219156 WP_152537873.1 NZ_CP084692:c1279422-1279003 [Enterobacter mori]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPDSPLLDIVCARISRYEASRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT219156 NZ_CP116191:c3607050-3606802 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W2Z921