Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4122763..4123304 Replicon chromosome
Accession NZ_CP084683
Organism Escherichia coli strain BL21(RP)

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag LGS58_RS19915 Protein ID WP_000615981.1
Coordinates 4123026..4123304 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LGS58_RS19910 Protein ID WP_000729704.1
Coordinates 4122763..4123023 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGS58_RS19890 (4118446) 4118446..4119231 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
LGS58_RS19895 (4119203) 4119203..4120915 + 1713 Protein_3933 flagellar biosynthesis protein FlhA -
LGS58_RS19900 (4121131) 4121131..4121628 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
LGS58_RS19905 (4121804) 4121804..4122577 - 774 WP_001353765.1 C40 family peptidase -
LGS58_RS19910 (4122763) 4122763..4123023 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LGS58_RS19915 (4123026) 4123026..4123304 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LGS58_RS19920 (4123460) 4123460..4124200 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LGS58_RS19925 (4124171) 4124171..4124938 - 768 WP_000333379.1 class II glutamine amidotransferase -
LGS58_RS19930 (4125144) 4125144..4125722 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4115143..4131807 16664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T219151 WP_000615981.1 NZ_CP084683:4123026-4123304 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T219151 NZ_CP116191:c1986338-1986231 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT219151 WP_000729704.1 NZ_CP084683:4122763-4123023 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT219151 NZ_CP116191:1986391-1986452 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References