Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 4122763..4123304 | Replicon | chromosome |
| Accession | NZ_CP084683 | ||
| Organism | Escherichia coli strain BL21(RP) | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | A0A140NAP5 |
| Locus tag | LGS58_RS19915 | Protein ID | WP_000615981.1 |
| Coordinates | 4123026..4123304 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | S1EWQ0 |
| Locus tag | LGS58_RS19910 | Protein ID | WP_000729704.1 |
| Coordinates | 4122763..4123023 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGS58_RS19890 (4118446) | 4118446..4119231 | - | 786 | WP_000207564.1 | putative lateral flagellar export/assembly protein LafU | - |
| LGS58_RS19895 (4119203) | 4119203..4120915 | + | 1713 | Protein_3933 | flagellar biosynthesis protein FlhA | - |
| LGS58_RS19900 (4121131) | 4121131..4121628 | - | 498 | WP_000006260.1 | REP-associated tyrosine transposase RayT | - |
| LGS58_RS19905 (4121804) | 4121804..4122577 | - | 774 | WP_001353765.1 | C40 family peptidase | - |
| LGS58_RS19910 (4122763) | 4122763..4123023 | + | 261 | WP_000729704.1 | type II toxin-antitoxin system antitoxin DinJ | Antitoxin |
| LGS58_RS19915 (4123026) | 4123026..4123304 | + | 279 | WP_000615981.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | Toxin |
| LGS58_RS19920 (4123460) | 4123460..4124200 | + | 741 | WP_001225679.1 | peptidoglycan meso-diaminopimelic acid protein amidase | - |
| LGS58_RS19925 (4124171) | 4124171..4124938 | - | 768 | WP_000333379.1 | class II glutamine amidotransferase | - |
| LGS58_RS19930 (4125144) | 4125144..4125722 | - | 579 | WP_000284050.1 | D-sedoheptulose 7-phosphate isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 4115143..4131807 | 16664 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10860.65 Da Isoelectric Point: 10.0702
>T219151 WP_000615981.1 NZ_CP084683:4123026-4123304 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T219151 NZ_CP116191:c1986338-1986231 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9391.77 Da Isoelectric Point: 5.0758
>AT219151 WP_000729704.1 NZ_CP084683:4122763-4123023 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT219151 NZ_CP116191:1986391-1986452 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4MMG | |
| PDB | 4MMJ | |
| PDB | 4ML2 | |
| PDB | 4ML0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6Z6 |