Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 238368..239095 Replicon chromosome
Accession NZ_CP084683
Organism Escherichia coli strain BL21(RP)

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LGS58_RS01240 Protein ID WP_000550189.1
Coordinates 238781..239095 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LGS58_RS01235 Protein ID WP_000560266.1
Coordinates 238368..238784 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGS58_RS01205 (233638) 233638..233916 + 279 WP_012775982.1 hypothetical protein -
LGS58_RS01210 (233918) 233918..234568 + 651 WP_001265279.1 DUF968 domain-containing protein -
LGS58_RS01215 (234586) 234586..234963 + 378 WP_001204787.1 antiterminator Q family protein -
LGS58_RS01220 (235119) 235119..235643 - 525 WP_000780584.1 lipocalin family protein -
LGS58_RS01225 (235836) 235836..235982 + 147 Protein_241 2-thiouracil desulfurase family protein -
LGS58_RS01230 (236305) 236305..238323 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LGS58_RS01235 (238368) 238368..238784 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LGS58_RS01240 (238781) 238781..239095 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LGS58_RS01245 (239379) 239379..240515 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LGS58_RS01250 (240600) 240600..241103 + 504 WP_001300832.1 M48 family metallopeptidase -
LGS58_RS01255 (241180) 241180..241872 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LGS58_RS01260 (241951) 241951..242937 + 987 WP_012775974.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T219131 WP_000550189.1 NZ_CP084683:c239095-238781 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T219131 NZ_CP116188:c2968387-2968133 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT219131 WP_000560266.1 NZ_CP084683:c238784-238368 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT219131 NZ_CP116188:c2968635-2968384 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References