Detailed information of TA system TA219082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 1755008..1756359
• Replicon: chromosome
• Accession: NZ_CP084655
• Organism: Pectobacterium brasiliense strain SR10

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: LGL97_RS07830
• Protein ID: WP_225584113.1
• Coordinates: 1755319..1756359 (+)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: LGL97_RS07825
• Protein ID: WP_205535564.1
• Coordinates: 1755008..1755322 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL97_RS07800 (LGL97_07800)	1750829..1751863	+	1035	WP_205535567.1	putative FMN-dependent luciferase-like monooxygenase	-
LGL97_RS07805 (LGL97_07805)	1751860..1752993	+	1134	WP_225584111.1	alkylhydroperoxidase domain protein	-
LGL97_RS07810 (LGL97_07810)	1753050..1753562	-	513	WP_225584151.1	phenolic acid decarboxylase	-
LGL97_RS07815 (LGL97_07815)	1753662..1754534	+	873	WP_039316648.1	LysR family transcriptional regulator	-
LGL97_RS07820 (LGL97_07820)	1754697..1755023	+	327	WP_225584112.1	helix-turn-helix domain-containing protein	-
LGL97_RS07825 (LGL97_07825)	1755008..1755322	+	315	WP_205535564.1	HipA N-terminal domain-containing protein	Antitoxin
LGL97_RS07830 (LGL97_07830)	1755319..1756359	+	1041	WP_225584113.1	HipA domain-containing protein	Toxin
LGL97_RS07835 (LGL97_07835)	1756419..1757042	-	624	WP_094431975.1	LysE family translocator	-
LGL97_RS07840 (LGL97_07840)	1757224..1757928	+	705	WP_225584115.1	SMI1/KNR4 family protein	-
LGL97_RS07845 (LGL97_07845)	1757998..1758753	+	756	WP_225584118.1	thiol:disulfide interchange protein DsbG	-
LGL97_RS07850 (LGL97_07850)	1758787..1759692	-	906	WP_048255948.1	siderophore-interacting protein	-
LGL97_RS07855 (LGL97_07855)	1759947..1760198	+	252	WP_010281712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LGL97_RS07860 (LGL97_07860)	1760195..1760482	+	288	WP_225584120.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (57-307)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39606.57 Da
• Isoelectric Point: 6.3999

>T219082 WP_225584113.1 NZ_CP084655:1755319-1756359 [Pectobacterium brasiliense]
MTRCRILLTPLTHEEEIATGYSRKGLKHLAGKMHTSPDLRFTRQQFMHDLPQAQKGMSISGYQPKIQLVLEEQAFTVVDH
QGLYILKPSPIEFPHLAENEHATMTLMARLGFAVPPHGLLRFKPEQADDEPEFAFVIKRFDRDEKTGHPLHQEQLDGAMG
IGEKFGKTHTDGRQYISYERLALFLSKHVNDNIVFKIDLFRRIAYAYMLGNNDMHLRNFGLMHLHSGQLMLAPIYDFVSV
APYPTYFSSCFMALPLLIQEEGDEALAPGFETAYGEYLGMDFILFGQRIGLSENLTKKLLADLTKEAECVETTYRDSFMP
ADAIETTLRCYRHRLNLMSILDAERI

Nucleotide

• Length: 1041 bp

>T219082 NZ_CP116176:c2646099-2645996 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11900.66 Da
• Isoelectric Point: 8.3628

>AT219082 WP_205535564.1 NZ_CP084655:1755008-1755322 [Pectobacterium brasiliense]
MRRQVKVYLYGVHIGQLSQDDKGYLFEYKESYIGPPLSLSLPIQTGTFRSRTLPPYFASLAPEGWLRRQYSELQHLDEKD
LFGILIQNGKNLIGAVQLIAEDKA

Nucleotide

• Length: 315 bp

>AT219082 NZ_CP116176:2645958-2646229 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT