Detailed information of TA system TA219069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4568582..4569306
• Replicon: chromosome
• Accession: NZ_CP084654
• Organism: Pectobacterium versatile strain SR12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7V8TLN0
• Locus tag: LGL95_RS20345
• Protein ID: WP_039504225.1
• Coordinates: 4568995..4569306 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGL95_RS20340
• Protein ID: WP_103861592.1
• Coordinates: 4568582..4568998 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL95_RS20315 (LGL95_20315)	4563759..4564289	+	531	WP_226305989.1	hypothetical protein	-
LGL95_RS20320 (LGL95_20320)	4564276..4564959	+	684	WP_264158860.1	pyridoxal-dependent decarboxylase	-
LGL95_RS20325 (LGL95_20325)	4565057..4565515	-	459	WP_039534732.1	hypothetical protein	-
LGL95_RS20330 (LGL95_20330)	4567284..4567511	-	228	WP_226306457.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
LGL95_RS20335 (LGL95_20335)	4567859..4568362	+	504	WP_109224920.1	hypothetical protein	-
LGL95_RS20340 (LGL95_20340)	4568582..4568998	-	417	WP_103861592.1	helix-turn-helix domain-containing protein	Antitoxin
LGL95_RS20345 (LGL95_20345)	4568995..4569306	-	312	WP_039504225.1	type II toxin-antitoxin system HigB family toxin	Toxin
LGL95_RS20350 (LGL95_20350)	4570738..4571031	-	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
LGL95_RS20355 (LGL95_20355)	4571032..4571949	-	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1e	-
LGL95_RS20360 (LGL95_20360)	4571977..4572624	-	648	WP_226306458.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
LGL95_RS20365 (LGL95_20365)	4572624..4573358	-	735	WP_226305991.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12406.28 Da
• Isoelectric Point: 10.0380

>T219069 WP_039504225.1 NZ_CP084654:c4569306-4568995 [Pectobacterium versatile]
MHVISRAPFDTATTQFPNQAAALADLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T219069 NZ_CP116169:c4321418-4321245 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15405.70 Da
• Isoelectric Point: 4.8037

>AT219069 WP_103861592.1 NZ_CP084654:c4568998-4568582 [Pectobacterium versatile]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT219069 NZ_CP116169:c4321742-4321446 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7V8TLN0