Detailed information of TA system TA219067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4465599..4466101
• Replicon: chromosome
• Accession: NZ_CP084654
• Organism: Pectobacterium versatile strain SR12

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LGL95_RS19895
• Protein ID: WP_107332237.1
• Coordinates: 4465847..4466101 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7W2E625
• Locus tag: LGL95_RS19890
• Protein ID: WP_039324900.1
• Coordinates: 4465599..4465850 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL95_RS19875 (LGL95_19875)	4462002..4462961	+	960	WP_029367586.1	ABC transporter permease	-
LGL95_RS19880 (LGL95_19880)	4462954..4463820	+	867	WP_125232223.1	ABC transporter permease	-
LGL95_RS19885 (LGL95_19885)	4463817..4465523	+	1707	WP_226305957.1	ABC transporter ATP-binding protein	-
LGL95_RS19890 (LGL95_19890)	4465599..4465850	+	252	WP_039324900.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LGL95_RS19895 (LGL95_19895)	4465847..4466101	+	255	WP_107332237.1	Txe/YoeB family addiction module toxin	Toxin
LGL95_RS19900 (LGL95_19900)	4466479..4467450	+	972	WP_116156383.1	reverse transcriptase domain-containing protein	-
LGL95_RS19905 (LGL95_19905)	4467447..4468775	+	1329	WP_125232221.1	AAA family ATPase	-
LGL95_RS19910 (LGL95_19910)	4468967..4469821	-	855	WP_040031929.1	helix-turn-helix domain-containing protein	-
LGL95_RS19915 (LGL95_19915)	4469846..4470685	-	840	WP_039493893.1	HTH-type transcriptional activator RhaS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10171.76 Da
• Isoelectric Point: 8.8560

>T219067 WP_107332237.1 NZ_CP084654:4465847-4466101 [Pectobacterium versatile]
VKLIWSEEAWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITGDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T219067 NZ_CP116169:c4151178-4150828 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9367.64 Da
• Isoelectric Point: 4.6119

>AT219067 WP_039324900.1 NZ_CP084654:4465599-4465850 [Pectobacterium versatile]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNGEERD
IIE

Nucleotide

• Length: 252 bp

>AT219067 NZ_CP116169:c4151423-4151172 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E625