Detailed information of TA system TA219049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 32255..32920
• Replicon: plasmid p16Y0180.1
• Accession: NZ_CP084653
• Organism: Yersinia ruckeri strain 16Y0180

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380S9H4
• Locus tag: LGL93_RS16940
• Protein ID: WP_038251855.1
• Coordinates: 32597..32920 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGL93_RS16935
• Protein ID: WP_004720859.1
• Coordinates: 32255..32551 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL93_RS16915 (LGL93_16960)	27601..29526	-	1926	WP_004720847.1	relaxase/mobilization nuclease domain-containing protein	-
LGL93_RS16920 (LGL93_16965)	29528..29872	-	345	WP_004720848.1	plasmid mobilization protein MobA	-
LGL93_RS16925 (LGL93_16970)	30141..30464	+	324	WP_004720850.1	hypothetical protein	-
LGL93_RS16930 (LGL93_16980)	31003..31821	-	819	WP_004720855.1	DUF932 domain-containing protein	-
LGL93_RS16935 (LGL93_16985)	32255..32551	-	297	WP_004720859.1	NadS family protein	Antitoxin
LGL93_RS16940 (LGL93_16990)	32597..32920	-	324	WP_038251855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL93_RS16945 (LGL93_16995)	33072..33305	+	234	WP_038251853.1	hypothetical protein	-
LGL93_RS16950 (LGL93_17000)	33313..33687	-	375	WP_004720865.1	hypothetical protein	-
LGL93_RS16955 (LGL93_17005)	33776..34471	-	696	WP_038251906.1	IS6 family transposase	-
LGL93_RS16960 (LGL93_17010)	35012..36211	+	1200	WP_038251850.1	AAA family ATPase	-
LGL93_RS16965 (LGL93_17015)	36220..37188	+	969	WP_004723386.1	ParB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103904	103904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.31 Da
• Isoelectric Point: 8.8371

>T219049 WP_038251855.1 NZ_CP084653:c32920-32597 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Nucleotide

• Length: 324 bp

>T219049 NZ_CP116168:90804-91220 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11027.77 Da
• Isoelectric Point: 6.7264

>AT219049 WP_004720859.1 NZ_CP084653:c32551-32255 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Nucleotide

• Length: 297 bp

>AT219049 NZ_CP116168:90577-90807 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A380S9H4