Detailed information of TA system TA219035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 85505..86170
• Replicon: plasmid p17Y0153.1
• Accession: NZ_CP084651
• Organism: Yersinia ruckeri strain 17Y0153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380S9H4
• Locus tag: LGL87_RS17810
• Protein ID: WP_038251855.1
• Coordinates: 85847..86170 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGL87_RS17805
• Protein ID: WP_004720859.1
• Coordinates: 85505..85801 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL87_RS17780 (LGL87_17805)	80851..82776	-	1926	WP_004720847.1	relaxase/mobilization nuclease domain-containing protein	-
LGL87_RS17785 (LGL87_17810)	82778..83122	-	345	WP_004720848.1	plasmid mobilization protein MobA	-
LGL87_RS17790 (LGL87_17815)	83391..83714	+	324	WP_004720850.1	hypothetical protein	-
LGL87_RS17795 (LGL87_17820)	83861..83977	-	117	WP_220272310.1	hypothetical protein	-
LGL87_RS17800 (LGL87_17825)	84253..85071	-	819	WP_004720855.1	DUF932 domain-containing protein	-
LGL87_RS17805 (LGL87_17830)	85505..85801	-	297	WP_004720859.1	NadS family protein	Antitoxin
LGL87_RS17810 (LGL87_17835)	85847..86170	-	324	WP_038251855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL87_RS17815 (LGL87_17840)	86322..86555	+	234	WP_038251853.1	hypothetical protein	-
LGL87_RS17820 (LGL87_17845)	86563..86937	-	375	WP_004720865.1	hypothetical protein	-
LGL87_RS17825 (LGL87_17850)	87026..87721	-	696	WP_038251906.1	IS6 family transposase	-
LGL87_RS17830 (LGL87_17855)	88262..89461	+	1200	WP_038251850.1	AAA family ATPase	-
LGL87_RS17835 (LGL87_17860)	89470..90438	+	969	WP_004723386.1	ParB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103903	103903

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.31 Da
• Isoelectric Point: 8.8371

>T219035 WP_038251855.1 NZ_CP084651:c86170-85847 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Nucleotide

• Length: 324 bp

>T219035 NZ_CP116166:894466-894873 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11027.77 Da
• Isoelectric Point: 6.7264

>AT219035 WP_004720859.1 NZ_CP084651:c85801-85505 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Nucleotide

• Length: 297 bp

>AT219035 NZ_CP116166:894219-894485 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A380S9H4