Detailed information of TA system TA219021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1..666
• Replicon: plasmid p17Y0155.1
• Accession: NZ_CP084649
• Organism: Yersinia ruckeri strain 17Y0155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380S9H4
• Locus tag: LGL92_RS17330
• Protein ID: WP_038251855.1
• Coordinates: 1..324 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGL92_RS17335
• Protein ID: WP_004720859.1
• Coordinates: 370..666 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL92_RS17330 (LGL92_17390)	1..324	+	324	WP_038251855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL92_RS17335 (LGL92_17395)	370..666	+	297	WP_004720859.1	NadS family protein	Antitoxin
LGL92_RS17340 (LGL92_17400)	1100..1918	+	819	WP_004720855.1	DUF932 domain-containing protein	-
LGL92_RS17345 (LGL92_17410)	2457..2780	-	324	WP_004720850.1	hypothetical protein	-
LGL92_RS17350 (LGL92_17415)	3049..3393	+	345	WP_004720848.1	plasmid mobilization protein MobA	-
LGL92_RS17355 (LGL92_17420)	3395..5320	+	1926	WP_004720847.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103903	103903

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.31 Da
• Isoelectric Point: 8.8371

>T219021 WP_038251855.1 NZ_CP084649:1-324 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Nucleotide

• Length: 324 bp

>T219021 NZ_CP116159:c3727825-3727427 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11027.77 Da
• Isoelectric Point: 6.7264

>AT219021 WP_004720859.1 NZ_CP084649:370-666 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Nucleotide

• Length: 297 bp

>AT219021 NZ_CP116159:c3728121-3727828 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A380S9H4