Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3505565..3506235 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BCG_RS16520 Protein ID WP_003899954.1
Coordinates 3505565..3505909 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BCG_RS16525 Protein ID WP_003899955.1
Coordinates 3505906..3506235 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS16490 3500638..3501498 + 861 WP_003416628.1 alpha/beta hydrolase -
BCG_RS16495 3501473..3501988 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
BCG_RS16500 3502228..3502521 + 294 WP_003416635.1 hypothetical protein -
BCG_RS16505 3502809..3504098 + 1290 WP_003416640.1 ATP-binding protein -
BCG_RS16510 3504445..3504879 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BCG_RS16515 3504882..3505334 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BCG_RS16520 3505565..3505909 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BCG_RS16525 3505906..3506235 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BCG_RS16530 3506773..3507120 + 348 WP_003899956.1 DUF2384 domain-containing protein -
BCG_RS16535 3507117..3507737 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
BCG_RS16540 3507897..3509162 - 1266 WP_003909837.1 hypothetical protein -
BCG_RS16545 3509330..3509539 + 210 WP_003416778.1 hypothetical protein -
BCG_RS16555 3509786..3510820 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T21897 WP_003899954.1 NC_008769:3505565-3505909 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T21897 NC_008769:3505565-3505909 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT21897 WP_003899955.1 NC_008769:3505906-3506235 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT21897 NC_008769:3505906-3506235 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References