Detailed information of TA system TA218945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 99820..100485
• Replicon: plasmid p17Y0189.1
• Accession: NZ_CP084640
• Organism: Yersinia ruckeri strain 17Y0189

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380S9H4
• Locus tag: LGL94_RS17855
• Protein ID: WP_038251855.1
• Coordinates: 100162..100485 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGL94_RS17850
• Protein ID: WP_004720859.1
• Coordinates: 99820..100116 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL94_RS17830 (LGL94_17820)	95166..97091	-	1926	WP_004720847.1	relaxase/mobilization nuclease domain-containing protein	-
LGL94_RS17835 (LGL94_17825)	97093..97437	-	345	WP_004720848.1	plasmid mobilization protein MobA	-
LGL94_RS17840 (LGL94_17830)	97706..98029	+	324	WP_004720850.1	hypothetical protein	-
LGL94_RS17845 (LGL94_17840)	98568..99386	-	819	WP_004720855.1	DUF932 domain-containing protein	-
LGL94_RS17850 (LGL94_17845)	99820..100116	-	297	WP_004720859.1	NadS family protein	Antitoxin
LGL94_RS17855 (LGL94_17850)	100162..100485	-	324	WP_038251855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL94_RS17860 (LGL94_17855)	100637..100870	+	234	WP_038251853.1	hypothetical protein	-
LGL94_RS17865 (LGL94_17860)	100878..101252	-	375	WP_004720865.1	hypothetical protein	-
LGL94_RS17870 (LGL94_17865)	101341..102036	-	696	WP_038251906.1	IS6 family transposase	-
LGL94_RS17875 (LGL94_17870)	102577..103776	+	1200	WP_038251850.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103901	103901
	-	flank	IS/Tn	-	-	101341..102036	695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.31 Da
• Isoelectric Point: 8.8371

>T218945 WP_038251855.1 NZ_CP084640:c100485-100162 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Nucleotide

• Length: 324 bp

>T218945 NZ_CP116136:3268205-3268591 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11027.77 Da
• Isoelectric Point: 6.7264

>AT218945 WP_004720859.1 NZ_CP084640:c100116-99820 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Nucleotide

• Length: 297 bp

>AT218945 NZ_CP116136:3268581-3268910 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A380S9H4