Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 56233..56864 Replicon plasmid p17Y0412.1
Accession NZ_CP084638
Organism Yersinia ruckeri strain 17Y0412

Toxin (Protein)


Gene name higB Uniprot ID A0A2R4NX45
Locus tag LGL85_RS17950 Protein ID WP_049615238.1
Coordinates 56574..56864 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LGL85_RS17945 Protein ID WP_049615233.1
Coordinates 56233..56562 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL85_RS17920 (LGL85_17970) 52460..52696 - 237 WP_073999971.1 hypothetical protein -
LGL85_RS17925 (LGL85_17975) 53004..53567 - 564 WP_234049629.1 helix-turn-helix transcriptional regulator -
LGL85_RS17930 (LGL85_17980) 53666..54070 - 405 WP_234049630.1 plasmid partitioning/stability family protein -
LGL85_RS17935 (LGL85_17985) 54077..55054 - 978 WP_234049631.1 plasmid segregation protein ParM -
LGL85_RS17940 (LGL85_17990) 55311..55811 - 501 WP_120806770.1 hypothetical protein -
LGL85_RS17945 (LGL85_17995) 56233..56562 - 330 WP_049615233.1 HigA family addiction module antitoxin Antitoxin
LGL85_RS17950 (LGL85_18000) 56574..56864 - 291 WP_049615238.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGL85_RS17955 (LGL85_18005) 57002..57823 - 822 WP_080991164.1 N-6 DNA methylase -
LGL85_RS17960 (LGL85_18010) 58197..58622 + 426 WP_234049632.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
LGL85_RS17965 (LGL85_18015) 58622..59878 + 1257 WP_234049633.1 translesion error-prone DNA polymerase V subunit UmuC -
LGL85_RS17970 (LGL85_18020) 59939..60295 - 357 WP_234049634.1 hypothetical protein -
LGL85_RS17975 (LGL85_18025) 60353..60580 - 228 WP_145544398.1 hypothetical protein -
LGL85_RS17980 (LGL85_18030) 60630..61250 - 621 WP_226723006.1 ParA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..83706 83706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(7-85)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11538.18 Da        Isoelectric Point: 9.3579

>T218931 WP_049615238.1 NZ_CP084638:c56864-56574 [Yersinia ruckeri]
MIKSFRDKNLELFYMESKRDRAIPATIERQLAKKLDMLAAAHNERDLFIPTSNYYKRLSGQFDGWSSMRVNIQWRLMFQW
KDGYAESIYLDPHQDT

Download         Length: 291 bp

>T218931 NZ_CP116136:254601-254708 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12630.54 Da        Isoelectric Point: 6.2374

>AT218931 WP_049615233.1 NZ_CP084638:c56562-56233 [Yersinia ruckeri]
MLDTTTREPTTVGEILQEEFLTPLAIGQEELADIMGVTRATVNRLCKGRRRLTVEEATLFSELFETTPEFWLNLQAAHDR
WEAREALQKHKRPPMRPIMEMLGRRISHA

Download         Length: 330 bp

>AT218931 NZ_CP116136:c254552-254489 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2R4NX45

Antitoxin

Source ID Structure

References