Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 108505..109162 | Replicon | plasmid p1 |
| Accession | NZ_CP084543 | ||
| Organism | Klebsiella michiganensis strain YL9 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | LD277_RS29640 | Protein ID | WP_000270043.1 |
| Coordinates | 108505..108855 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LD277_RS29645 | Protein ID | WP_000124640.1 |
| Coordinates | 108860..109162 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LD277_RS29605 (LD277_29640) | 104979..105476 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| LD277_RS29610 (LD277_29645) | 105479..105967 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| LD277_RS29615 (LD277_29650) | 106064..106399 | + | 336 | WP_000683476.1 | hypothetical protein | - |
| LD277_RS29620 (LD277_29655) | 106414..106884 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| LD277_RS29625 (LD277_29660) | 106877..107248 | - | 372 | WP_000516916.1 | hypothetical protein | - |
| LD277_RS29630 (LD277_29665) | 107259..107453 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| LD277_RS29635 (LD277_29670) | 107794..108342 | - | 549 | WP_001061574.1 | transcriptional regulator | - |
| LD277_RS29640 (LD277_29675) | 108505..108855 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LD277_RS29645 (LD277_29680) | 108860..109162 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| LD277_RS29650 (LD277_29685) | 109189..109482 | - | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| LD277_RS29655 (LD277_29690) | 109570..109842 | - | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| LD277_RS29660 (LD277_29695) | 109900..110427 | - | 528 | WP_001236377.1 | thermonuclease family protein | - |
| LD277_RS29665 (LD277_29700) | 110658..111515 | - | 858 | WP_001167032.1 | hypothetical protein | - |
| LD277_RS29670 (LD277_29705) | 111502..111732 | - | 231 | WP_000972663.1 | hypothetical protein | - |
| LD277_RS29675 (LD277_29710) | 111732..112250 | - | 519 | WP_000210756.1 | nitrite reductase | - |
| LD277_RS29680 (LD277_29715) | 112247..112693 | - | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| LD277_RS29685 (LD277_29720) | 112693..113052 | - | 360 | WP_000422768.1 | hypothetical protein | - |
| LD277_RS29690 (LD277_29725) | 113109..113537 | - | 429 | WP_000591074.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaVEB-3 / qacE / sul1 / aph(3'')-Ib / sul2 | - | 1..145051 | 145051 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T218866 WP_000270043.1 NZ_CP084543:108505-108855 [Klebsiella michiganensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T218866 NZ_CP116113:7974-8390 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT218866 WP_000124640.1 NZ_CP084543:108860-109162 [Klebsiella michiganensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT218866 NZ_CP116113:7747-7977 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|