218851

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40171..40821 Replicon chromosome
Accession NZ_CP084542
Organism Klebsiella michiganensis strain YL9

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag LD277_RS00195 Protein ID WP_014227314.1
Coordinates 40171..40512 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag LD277_RS00200 Protein ID WP_014227313.1
Coordinates 40522..40821 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD277_RS00180 (LD277_00180) 36434..37753 + 1320 WP_014227316.1 MFS transporter -
LD277_RS00185 (LD277_00185) 37899..39290 + 1392 WP_004107098.1 hexose-6-phosphate:phosphate antiporter -
LD277_RS00190 (LD277_00190) 39468..39920 + 453 WP_014839842.1 DUF1198 domain-containing protein -
LD277_RS00195 (LD277_00195) 40171..40512 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LD277_RS00200 (LD277_00200) 40522..40821 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
LD277_RS00205 (LD277_00205) 40940..42121 + 1182 WP_014839841.1 purine ribonucleoside efflux pump NepI -
LD277_RS00210 (LD277_00210) 42154..42963 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
LD277_RS00215 (LD277_00215) 42973..44076 - 1104 WP_042934427.1 2-aminoethylphosphonate--pyruvate transaminase -
LD277_RS00220 (LD277_00220) 44217..44936 + 720 WP_014839839.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T218851 WP_014227314.1 NZ_CP084542:40171-40512 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T218851 NZ_CP116110:1799991-1800245 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT218851 WP_014227313.1 NZ_CP084542:40522-40821 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT218851 NZ_CP116110:1799743-1799994 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References