Detailed information of TA system TA218817

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-istR/-
• Location: 4554904..4555325
• Replicon: chromosome
• Accession: NZ_CP084529
• Organism: Escherichia coli strain Origami2-DE3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LFW28_RS22175
• Protein ID: WP_096937776.1
• Coordinates: 4554904..4555029 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	istR
Locus tag	-
Coordinates	4555127..4555325 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFW28_RS22130 (4549952)	4549952..4550257	+	306	WP_010892536.1	hypothetical protein	-
LFW28_RS22135 (4550334)	4550334..4551017	+	684	WP_000085939.1	DNA methylase	-
LFW28_RS22140 (4551018)	4551018..4551239	+	222	WP_001104904.1	hypothetical protein	-
LFW28_RS22145 (4551133)	4551133..4551687	+	555	WP_001358893.1	DUF1380 family protein	-
LFW28_RS22150 (4551733)	4551733..4552509	+	777	WP_001005032.1	hypothetical protein	-
LFW28_RS22155 (4552923)	4552923..4553348	+	426	WP_001198920.1	antirestriction protein	-
LFW28_RS22160 (4553395)	4553395..4553817	+	423	WP_000271685.1	DUF1380 family protein	-
LFW28_RS22165 (4553814)	4553814..4554005	+	192	WP_001027495.1	hypothetical protein	-
LFW28_RS22170 (4554334)	4554334..4554603	+	270	Protein_4354	hypothetical protein	-
LFW28_RS22175 (4554904)	4554904..4555029	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
LFW28_RS22180 (4554971)	4554971..4555120	-	150	Protein_4356	DUF5431 family protein	-
- (4555127)	4555127..4555325	-	199	NuclAT_1	-	-
- (4555127)	4555127..4555325	-	199	NuclAT_1	-	-
- (4555127)	4555127..4555325	-	199	NuclAT_1	-	-
- (4555127)	4555127..4555325	-	199	NuclAT_1	-	-
LFW28_RS22185 (4555294)	4555294..4556056	-	763	Protein_4357	plasmid SOS inhibition protein A	-
LFW28_RS22190 (4556053)	4556053..4556487	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
LFW28_RS22195 (4556542)	4556542..4558500	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
LFW28_RS22200 (4558566)	4558566..4558799	-	234	WP_000005990.1	DUF905 family protein	-
LFW28_RS22205 (4558856)	4558856..4559395	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
LFW28_RS22210 (4559713)	4559713..4559926	+	214	Protein_4362	hypothetical protein	-
LFW28_RS22215 (4559927)	4559927..4560049	+	123	Protein_4363	protein ren	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T218817 WP_096937776.1 NZ_CP084529:c4555029-4554904 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T218817 NZ_CP116104:96270-96686 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT218817 NZ_CP084529:4555127-4555325 [Escherichia coli]
GCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGCAACAAACCACCTTCACGTC
ATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATCCCTGCACGCTGACCACACTCACTTTTCCTGAAAAT
AACCCGCTCATTCAGGCTGTTTACGGGTAATCTGTGTGA

Structures

Antitoxin