Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4299421..4299962 Replicon chromosome
Accession NZ_CP084529
Organism Escherichia coli strain Origami2-DE3

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LFW28_RS20680 Protein ID WP_000615983.1
Coordinates 4299684..4299962 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LFW28_RS20675 Protein ID WP_000729703.1
Coordinates 4299421..4299681 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFW28_RS20655 (4295097) 4295097..4295882 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LFW28_RS20660 (4295854) 4295854..4297566 + 1713 Protein_4058 flagellar biosynthesis protein FlhA -
LFW28_RS20665 (4297790) 4297790..4298287 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LFW28_RS20670 (4298462) 4298462..4299211 - 750 WP_000056849.1 C40 family peptidase -
LFW28_RS20675 (4299421) 4299421..4299681 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LFW28_RS20680 (4299684) 4299684..4299962 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LFW28_RS20685 (4300118) 4300118..4300858 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LFW28_RS20690 (4300829) 4300829..4301596 - 768 WP_000333380.1 class II glutamine amidotransferase -
LFW28_RS20695 (4302039) 4302039..4302770 + 732 WP_000388269.1 protein YagJ -
LFW28_RS20700 (4302861) 4302861..4303487 - 627 WP_000860996.1 inovirus Gp2 family protein -
LFW28_RS20705 (4303759) 4303759..4304457 - 699 WP_001214248.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 4269686..4334803 65117


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T218815 WP_000615983.1 NZ_CP084529:4299684-4299962 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T218815 NZ_CP116103:4934758-4935069 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT218815 WP_000729703.1 NZ_CP084529:4299421-4299681 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT218815 NZ_CP116103:4935070-4935360 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References