Detailed information of TA system TA218798

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 2178825..2179237
• Replicon: chromosome
• Accession: NZ_CP084529
• Organism: Escherichia coli strain Origami2-DE3

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LFW28_RS10380
• Protein ID: WP_000132601.1
• Coordinates: 2178896..2179237 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	2178825..2178901 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFW28_RS10370 (2175688)	2175688..2177277	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
LFW28_RS10375 (2177274)	2177274..2178668	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (2178825)	2178825..2178901	-	77	NuclAT_16	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_16	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_16	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_16	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_17	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_17	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_17	-	Antitoxin
- (2178825)	2178825..2178901	-	77	NuclAT_17	-	Antitoxin
LFW28_RS10380 (2178896)	2178896..2179237	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
LFW28_RS10385 (2179399)	2179399..2180778	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LFW28_RS10390 (2180778)	2180778..2181824	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T218798 WP_000132601.1 NZ_CP084529:2178896-2179237 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T218798 NZ_CP116103:c575321-575064 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT218798 NZ_CP084529:c2178901-2178825 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin