Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1223136..1223863 Replicon chromosome
Accession NZ_CP084529
Organism Escherichia coli strain Origami2-DE3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LFW28_RS05900 Protein ID WP_000550189.1
Coordinates 1223549..1223863 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LFW28_RS05895 Protein ID WP_000560266.1
Coordinates 1223136..1223552 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFW28_RS05885 (1218296) 1218296..1220647 + 2352 WP_000695487.1 alpha-glucosidase -
LFW28_RS05890 (1221073) 1221073..1223091 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LFW28_RS05895 (1223136) 1223136..1223552 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LFW28_RS05900 (1223549) 1223549..1223863 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LFW28_RS05905 (1224147) 1224147..1225283 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LFW28_RS05910 (1225368) 1225368..1225871 + 504 WP_001333820.1 M48 family metallopeptidase -
LFW28_RS05915 (1225948) 1225948..1226640 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LFW28_RS05920 (1226719) 1226719..1227705 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T218794 WP_000550189.1 NZ_CP084529:c1223863-1223549 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T218794 NZ_CP116101:134895-135311 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT218794 WP_000560266.1 NZ_CP084529:c1223552-1223136 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT218794 NZ_CP116101:134668-134898 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References