Detailed information of TA system TA218743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 80630..81304
• Replicon: plasmid pIncFIIK_FIA
• Accession: NZ_CP084504
• Organism: Klebsiella pneumoniae strain MI01_K

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: KRU50_RS27180
• Protein ID: WP_032720638.1
• Coordinates: 80981..81304 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KRU50_RS27175
• Protein ID: WP_032720637.1
• Coordinates: 80630..80932 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRU50_RS27135 (KRU50_27165)	75823..76395	+	573	WP_044266753.1	hypothetical protein	-
KRU50_RS27140 (KRU50_27170)	76426..76920	+	495	WP_044266755.1	hypothetical protein	-
KRU50_RS27145 (KRU50_27175)	77223..78143	+	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
KRU50_RS27150 (KRU50_27180)	78155..78967	+	813	WP_223203129.1	TSUP family transporter	-
KRU50_RS27155 (KRU50_27185)	78969..79541	+	573	WP_032720635.1	GNAT family N-acetyltransferase	-
KRU50_RS27160 (KRU50_27190)	79646..80029	+	384	WP_032720636.1	hypothetical protein	-
KRU50_RS27165 (KRU50_27195)	80026..80232	+	207	WP_153591439.1	hypothetical protein	-
KRU50_RS27170 (KRU50_27200)	80420..80587	+	168	WP_153591440.1	hypothetical protein	-
KRU50_RS27175 (KRU50_27205)	80630..80932	-	303	WP_032720637.1	NadS family protein	Antitoxin
KRU50_RS27180 (KRU50_27210)	80981..81304	-	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KRU50_RS27185 (KRU50_27215)	81482..81887	-	406	Protein_91	DUF4113 domain-containing protein	-
KRU50_RS27190 (KRU50_27220)	81865..82292	+	428	Protein_92	DNA-binding protein	-
KRU50_RS27195 (KRU50_27225)	82361..82963	-	603	Protein_93	transposase	-
KRU50_RS27200 (KRU50_27230)	83464..83787	-	324	WP_019725650.1	hypothetical protein	-
KRU50_RS27205 (KRU50_27235)	83860..84855	-	996	WP_019725651.1	hypothetical protein	-
KRU50_RS27210 (KRU50_27240)	85152..85802	-	651	WP_015065500.1	SOS response-associated peptidase	-
KRU50_RS27215 (KRU50_27245)	85849..86247	-	399	Protein_97	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrB1	-	1..149924	149924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T218743 WP_032720638.1 NZ_CP084504:c81304-80981 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T218743 NZ_CP116090:45153-45305 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT218743 WP_032720637.1 NZ_CP084504:c80932-80630 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT218743 NZ_CP116090:c45101-45041 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5