Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 7789..8516 Replicon plasmid pIncFIBK_IncFII
Accession NZ_CP084503
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag KRU50_RS25370 Protein ID WP_004118600.1
Coordinates 7789..8100 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag KRU50_RS25375 Protein ID WP_000990392.1
Coordinates 8097..8516 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS25350 (KRU50_25380) 4303..5022 + 720 WP_001102107.1 (Fe-S)-binding protein -
KRU50_RS25355 (KRU50_25385) 5033..6460 + 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
KRU50_RS25360 (KRU50_25390) 6453..7148 + 696 WP_004118595.1 lactate utilization protein C -
KRU50_RS25365 (KRU50_25395) 7198..7578 - 381 Protein_6 gluconate permease -
KRU50_RS25370 (KRU50_25400) 7789..8100 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
KRU50_RS25375 (KRU50_25405) 8097..8516 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
KRU50_RS25380 (KRU50_25410) 8553..9755 - 1203 WP_003031541.1 hypothetical protein -
KRU50_RS25385 (KRU50_25415) 9748..10077 - 330 WP_004118613.1 hypothetical protein -
KRU50_RS25390 (KRU50_25420) 10074..10733 - 660 WP_000078540.1 hypothetical protein -
KRU50_RS25395 (KRU50_25425) 11157..11465 - 309 WP_020324596.1 Ref family recombination enhancement nuclease -
KRU50_RS25400 (KRU50_25430) 11633..11773 - 141 WP_004118614.1 hypothetical protein -
KRU50_RS25405 (KRU50_25435) 12066..12419 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KRU50_RS25410 (KRU50_25440) 12467..12829 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / qnrS1 - 1..251955 251955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T218739 WP_004118600.1 NZ_CP084503:7789-8100 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T218739 NZ_CP116089:7606-8022 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT218739 WP_000990392.1 NZ_CP084503:8097-8516 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT218739 NZ_CP116089:7379-7609 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References