Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 371398..372044 Replicon chromosome
Accession NZ_CP084502
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag KRU50_RS01715 Protein ID WP_016529833.1
Coordinates 371398..371745 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A231WWU8
Locus tag KRU50_RS01720 Protein ID WP_004174017.1
Coordinates 371745..372044 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS01705 (367324) 367324..368757 + 1434 WP_002920564.1 glycogen synthase GlgA -
KRU50_RS01710 (368775) 368775..371222 + 2448 WP_002920561.1 glycogen phosphorylase -
KRU50_RS01715 (371398) 371398..371745 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KRU50_RS01720 (371745) 371745..372044 + 300 WP_004174017.1 XRE family transcriptional regulator Antitoxin
KRU50_RS01725 (372107) 372107..373615 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KRU50_RS01730 (373820) 373820..374149 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KRU50_RS01735 (374200) 374200..375030 + 831 WP_032428425.1 rhomboid family intramembrane serine protease GlpG -
KRU50_RS01740 (375080) 375080..375838 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T218722 WP_016529833.1 NZ_CP084502:371398-371745 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T218722 NZ_CP116088:345250-345353 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10844.64 Da        Isoelectric Point: 10.1464

>AT218722 WP_004174017.1 NZ_CP084502:371745-372044 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT218722 NZ_CP116088:c345391-345120 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A231WWU8

References