Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 344833..345419 Replicon chromosome
Accession NZ_CP084502
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name doc Uniprot ID A0A331ME34
Locus tag KRU50_RS01605 Protein ID WP_025861226.1
Coordinates 345051..345419 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KRU50_RS01600 Protein ID WP_004174006.1
Coordinates 344833..345054 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS01580 (340989) 340989..341915 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KRU50_RS01585 (341912) 341912..343189 + 1278 WP_258897632.1 branched chain amino acid ABC transporter permease LivM -
KRU50_RS01590 (343186) 343186..343953 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KRU50_RS01595 (343955) 343955..344668 + 714 WP_085920978.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KRU50_RS01600 (344833) 344833..345054 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KRU50_RS01605 (345051) 345051..345419 + 369 WP_025861226.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KRU50_RS01610 (345692) 345692..347008 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KRU50_RS01615 (347115) 347115..348002 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KRU50_RS01620 (347999) 347999..348844 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KRU50_RS01625 (348846) 348846..349916 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 341912..350653 8741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13551.98 Da        Isoelectric Point: 8.6410

>T218721 WP_025861226.1 NZ_CP084502:345051-345419 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T218721 NZ_CP116088:114503-114757 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT218721 WP_004174006.1 NZ_CP084502:344833-345054 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT218721 NZ_CP116088:114255-114506 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331ME34

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References