Detailed information of TA system TA218717

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 162837..163090
• Replicon: plasmid p1632-1
• Accession: NZ_CP084498
• Organism: Klebsiella pneumoniae strain 1632

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LDL62_RS28255
• Protein ID: WP_001312851.1
• Coordinates: 162941..163090 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	162837..162896 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL62_RS28220 (158615)	158615..159319	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LDL62_RS28225 (159344)	159344..159544	+	201	WP_072354025.1	hypothetical protein	-
LDL62_RS28230 (159564)	159564..160310	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LDL62_RS28235 (160365)	160365..160925	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LDL62_RS28240 (161057)	161057..161257	+	201	WP_015059022.1	hypothetical protein	-
LDL62_RS28245 (161643)	161643..162242	+	600	WP_032083981.1	PIN domain-containing protein	-
LDL62_RS28250 (162304)	162304..162636	+	333	WP_152916585.1	hypothetical protein	-
- (162837)	162837..162896	-	60	NuclAT_1	-	Antitoxin
- (162837)	162837..162896	-	60	NuclAT_1	-	Antitoxin
- (162837)	162837..162896	-	60	NuclAT_1	-	Antitoxin
- (162837)	162837..162896	-	60	NuclAT_1	-	Antitoxin
LDL62_RS28255 (162941)	162941..163090	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LDL62_RS28260 (163374)	163374..163622	+	249	WP_000083837.1	replication regulatory protein RepA	-
LDL62_RS28265 (163737)	163737..163921	+	185	Protein_219	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..163933	163933
	-	flank	IS/Tn	-	-	158615..159319	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T218717 WP_001312851.1 NZ_CP084498:162941-163090 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T218717 NZ_CP116085:c4470646-4470473 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT218717 NZ_CP084498:c162896-162837 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin