Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1733793..1734420 Replicon chromosome
Accession NZ_CP084433
Organism Edwardsiella piscicida strain S07-534

Toxin (Protein)


Gene name higB Uniprot ID A0A411H7J1
Locus tag DCF40_RS08055 Protein ID WP_015871332.1
Coordinates 1734091..1734420 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID C5BBM8
Locus tag DCF40_RS08050 Protein ID WP_015871333.1
Coordinates 1733793..1734101 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DCF40_RS08015 (DCF40_08015) 1728904..1729164 + 261 WP_069578552.1 hypothetical protein -
DCF40_RS08020 (DCF40_08020) 1729366..1730304 + 939 WP_069578554.1 helix-turn-helix domain-containing protein -
DCF40_RS08025 (DCF40_08025) 1730301..1731683 + 1383 WP_069578555.1 replicative DNA helicase -
DCF40_RS08030 (DCF40_08030) 1731713..1732060 + 348 WP_069578556.1 hypothetical protein -
DCF40_RS08035 (DCF40_08035) 1732170..1732814 + 645 WP_069578557.1 DUF1367 family protein -
DCF40_RS08040 (DCF40_08040) 1732817..1733119 + 303 WP_069578558.1 DUF1364 family protein -
DCF40_RS08045 (DCF40_08045) 1733411..1733656 + 246 WP_069578559.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
DCF40_RS08050 (DCF40_08050) 1733793..1734101 - 309 WP_015871333.1 helix-turn-helix transcriptional regulator Antitoxin
DCF40_RS08055 (DCF40_08055) 1734091..1734420 - 330 WP_015871332.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DCF40_RS08060 (DCF40_08060) 1735828..1736589 + 762 WP_071890285.1 antA/AntB antirepressor family protein -
DCF40_RS08065 (DCF40_08065) 1736823..1737803 - 981 WP_071890287.1 hypothetical protein -
DCF40_RS08070 (DCF40_08070) 1737800..1738321 - 522 WP_133176305.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1695641..1774137 78496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12771.78 Da        Isoelectric Point: 9.6247

>T218623 WP_015871332.1 NZ_CP084433:c1734420-1734091 [Edwardsiella piscicida]
MNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGIARVFYCTLTGKRIVML
HSFIKKTQKTPSAERKKAETRMKEVKHGW

Download         Length: 330 bp

>T218623 NZ_CP116067:865521-865751 [Escherichia coli]
ATGCGTATTTACGGAGCTAATCTTGGTTCCCCGCATACAGAGGCATTTGGCGACGGTTTGTTTGAAATCCGCCTTAAGGG
CTCGGAAGGCATAGGGCGTGTTTTTTACTGCACGCTAAAAGGAAAGCGAATCATTATGTTGCATAGCTTTGTAAAGAAAA
CGCAAAAAACTCCACCTGCCGAGCTTAGAAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11207.86 Da        Isoelectric Point: 8.5371

>AT218623 WP_015871333.1 NZ_CP084433:c1734101-1733793 [Edwardsiella piscicida]
MAGKRTPPTMTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKATAITRMESNLASGQSG
PSFATLKKFAHATGKKLQIRFV

Download         Length: 309 bp

>AT218623 NZ_CP116067:865741-866049 [Escherichia coli]
ATGACTGGTAAACGTACCCCACCTACCATGACACACGATGAAATGGTAGAAAAAATGTTGTCTAACCCCGCAGTAAAAGC
AGAATACGACGCCATCGCCGATGAATTCGCACTACTTGATGAAATGCTGGCAGCACGAAAAGAAGCTGGCTTAACCCAGG
CTGAAGTTGCTGAACGTATGGGAACAAAAGCGACTGCGATAACCAGAATGGAAAGCAATCTTGCATCAGGTACAAGCGGC
CCATCATTTGCCACACTGAAAAAATTCGCCCGTGCTACAGGGAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A411H7J1

Antitoxin

Source ID Structure
AlphaFold DB C5BBM8

References