Detailed information of TA system TA218601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1654994..1655621
• Replicon: chromosome
• Accession: NZ_CP084432
• Organism: Edwardsiella tarda strain AL98-87

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2A7U6J2
• Locus tag: DCF75_RS07630
• Protein ID: WP_005294220.1
• Coordinates: 1655292..1655621 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DCF75_RS07625
• Protein ID: WP_005286394.1
• Coordinates: 1654994..1655302 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCF75_RS07595 (DCF75_07595)	1650407..1651789	+	1383	WP_005286412.1	replicative DNA helicase	-
DCF75_RS07600 (DCF75_07600)	1651817..1652164	+	348	WP_098143178.1	hypothetical protein	-
DCF75_RS07605 (DCF75_07605)	1652274..1652918	+	645	WP_116605177.1	DUF1367 family protein	-
DCF75_RS07610 (DCF75_07610)	1652921..1653223	+	303	WP_116605176.1	DUF1364 family protein	-
DCF75_RS07615 (DCF75_07615)	1653315..1654010	+	696	WP_116605175.1	hypothetical protein	-
DCF75_RS07620 (DCF75_07620)	1654387..1654899	-	513	WP_226086039.1	DUF2442 domain-containing protein	-
DCF75_RS07625 (DCF75_07625)	1654994..1655302	-	309	WP_005286394.1	helix-turn-helix transcriptional regulator	Antitoxin
DCF75_RS07630 (DCF75_07630)	1655292..1655621	-	330	WP_005294220.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DCF75_RS07635 (DCF75_07635)	1656069..1656851	+	783	WP_116605686.1	Rha family transcriptional regulator	-
DCF75_RS07640 (DCF75_07640)	1657071..1657418	-	348	WP_116605684.1	STAS-like domain-containing protein	-
DCF75_RS07645 (DCF75_07645)	1657418..1658395	-	978	WP_116605682.1	hypothetical protein	-
DCF75_RS07650 (DCF75_07650)	1658680..1659303	+	624	WP_226086041.1	hypothetical protein	-
DCF75_RS07655 (DCF75_07655)	1659668..1660015	+	348	WP_116605688.1	phage holin, lambda family	-
DCF75_RS07660 (DCF75_07660)	1660018..1660569	+	552	WP_097364620.1	putative peptidoglycan-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1624135..1678697	54562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12799.83 Da
• Isoelectric Point: 9.8421

>T218601 WP_005294220.1 NZ_CP084432:c1655621-1655292 [Edwardsiella tarda]
MNYTIEYYSEEVRLEVDRLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGIARVFYCTLTGKRIVML
HSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 330 bp

>T218601 NZ_CP116046:c960022-959919 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11233.90 Da
• Isoelectric Point: 8.5371

>AT218601 WP_005286394.1 NZ_CP084432:c1655302-1654994 [Edwardsiella tarda]
MAGKRTPPTMTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKPTAITRMESNLASGQSG
PSFATLKKFAHATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT218601 NZ_CP116046:959881-960152 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A2A7U6J2