Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 61239..61896 | Replicon | plasmid p2 |
| Accession | NZ_CP084417 | ||
| Organism | Klebsiella michiganensis strain YL6-1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | LDP80_RS30065 | Protein ID | WP_000270043.1 |
| Coordinates | 61239..61589 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LDP80_RS30070 | Protein ID | WP_000124640.1 |
| Coordinates | 61594..61896 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LDP80_RS30030 (LDP80_30070) | 57713..58210 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| LDP80_RS30035 (LDP80_30075) | 58213..58701 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| LDP80_RS30040 (LDP80_30080) | 58798..59133 | + | 336 | WP_000683476.1 | hypothetical protein | - |
| LDP80_RS30045 (LDP80_30085) | 59148..59618 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| LDP80_RS30050 (LDP80_30090) | 59611..59982 | - | 372 | WP_000516916.1 | hypothetical protein | - |
| LDP80_RS30055 (LDP80_30095) | 59993..60187 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| LDP80_RS30060 (LDP80_30100) | 60528..61076 | - | 549 | WP_001061574.1 | transcriptional regulator | - |
| LDP80_RS30065 (LDP80_30105) | 61239..61589 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LDP80_RS30070 (LDP80_30110) | 61594..61896 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| LDP80_RS30075 (LDP80_30115) | 61923..62216 | - | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| LDP80_RS30080 (LDP80_30120) | 62304..62576 | - | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| LDP80_RS30085 (LDP80_30125) | 62634..63161 | - | 528 | WP_001236377.1 | thermonuclease family protein | - |
| LDP80_RS30090 (LDP80_30130) | 63392..64249 | - | 858 | WP_001167032.1 | hypothetical protein | - |
| LDP80_RS30095 (LDP80_30135) | 64236..64466 | - | 231 | WP_000972663.1 | hypothetical protein | - |
| LDP80_RS30100 (LDP80_30140) | 64466..64984 | - | 519 | WP_000210756.1 | nitrite reductase | - |
| LDP80_RS30105 (LDP80_30145) | 64981..65427 | - | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| LDP80_RS30110 (LDP80_30150) | 65427..65786 | - | 360 | WP_000422768.1 | hypothetical protein | - |
| LDP80_RS30115 (LDP80_30155) | 65843..66271 | - | 429 | WP_000591074.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaVEB-3 / qacE / sul1 / aph(3'')-Ib / sul2 | - | 1..145051 | 145051 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T218550 WP_000270043.1 NZ_CP084417:61239-61589 [Klebsiella michiganensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T218550 NZ_CP116037:c48660-48244 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT218550 WP_000124640.1 NZ_CP084417:61594-61896 [Klebsiella michiganensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT218550 NZ_CP116037:c48887-48657 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|