Detailed information of TA system TA218477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8039..8679
• Replicon: plasmid unnamed1
• Accession: NZ_CP084344
• Organism: Yersinia pestis strain 20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDH65_RS20910
• Protein ID: WP_002225538.1
• Coordinates: 8039..8380 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: LDH65_RS20915
• Protein ID: WP_002213446.1
• Coordinates: 8380..8679 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH65_RS20895 (LDH65_20875)	3765..3917	-	153	Protein_4	Rop family plasmid primer RNA-binding protein	-
LDH65_RS20900 (LDH65_20880)	4972..5749	-	778	Protein_5	IS21-like element helper ATPase IstB	-
LDH65_RS20905 (LDH65_20885)	5752..6773	-	1022	Protein_6	IS21-like element IS100 family transposase	-
LDH65_RS20910 (LDH65_20890)	8039..8380	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDH65_RS20915 (LDH65_20895)	8380..8679	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
LDH65_RS20920 (LDH65_20900)	8866..9804	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
LDH65_RS20925 (LDH65_20905)	10047..10352	-	306	WP_002221218.1	hypothetical protein	-
LDH65_RS20930 (LDH65_20910)	10580..11653	+	1074	WP_002218509.1	pesticin	-
LDH65_RS20935 (LDH65_20915)	11688..12083	-	396	WP_223661135.1	pesticin immunity protein	-
LDH65_RS20940 (LDH65_20920)	13349..13543	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / pla	1..19159	19159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T218477 WP_002225538.1 NZ_CP084344:8039-8380 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T218477 NZ_CP115992:c1885496-1885155 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT218477 WP_002213446.1 NZ_CP084344:8380-8679 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT218477 NZ_CP115992:c1885726-1885496 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016