Detailed information of TA system TA218463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7810..8450
• Replicon: plasmid unnamed3
• Accession: NZ_CP084342
• Organism: Yersinia pestis strain 94

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDH63_RS21930
• Protein ID: WP_002225538.1
• Coordinates: 7810..8151 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: LDH63_RS21935
• Protein ID: WP_002213446.1
• Coordinates: 8151..8450 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH63_RS21915 (LDH63_21895)	3512..3676	-	165	WP_002422899.1	Rop family plasmid primer RNA-binding protein	-
LDH63_RS21920 (LDH63_21900)	4743..5525	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
LDH63_RS21925 (LDH63_21905)	5522..6544	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
LDH63_RS21930 (LDH63_21910)	7810..8151	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDH63_RS21935 (LDH63_21915)	8151..8450	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
LDH63_RS21940 (LDH63_21920)	8637..9575	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
LDH63_RS21945 (LDH63_21925)	9818..10123	-	306	WP_002221218.1	hypothetical protein	-
LDH63_RS21950 (LDH63_21930)	10352..11425	+	1074	WP_002218509.1	pesticin	-
LDH63_RS21955 (LDH63_21935)	11460..11855	-	396	WP_223661135.1	pesticin immunity protein	-
LDH63_RS21960 (LDH63_21940)	13121..13315	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla	1..18011	18011

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T218463 WP_002225538.1 NZ_CP084342:7810-8151 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T218463 NZ_CP115970:7987-8250 [Wohlfahrtiimonas chitiniclastica]
ATGAATAGTAGAAATATCGAATGGACTAGTAATTCTTGGGATGAATATATTTATTGGCAAACACAAGATAAAAAAATCCT
TAAACGTATTAATTCTTTAATTAAAGAGTGTCAAAGAACACCTTTTGAAGGTACTGGTAAACCAGAACCTTTAAAAGCTA
ATCTTTCTGGCTTTTGGAGCAGAAGAATAGATGAAAAACATCGCTTAGTATATGAAGTCACAGATGAAAAAATATCCATT
GTTCAATGTAGATTTCATTACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT218463 WP_002213446.1 NZ_CP084342:8151-8450 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT218463 NZ_CP115970:7737-7994 [Wohlfahrtiimonas chitiniclastica]
ATGCATGTATTCACATATACTGATGCTCGTAATAACTTAAAATCGGTTTTAGATAAAGTAGTTGATGATGCTGACGTTGC
ATTTATCACCAGAAAAGAAGGTGGCCATGCTGTGGTTATGGGCCAAGATCATTATAATAGTTTAATGGAAACACTTTACC
TTTTATCTTCACCAAATAATACTAATCGTTTGAATGAATCAATTGCTCAATTACGATCAGGTCAAGTTACAATAAGAGAA
TTAATCGAGGATGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A080J016