Detailed information of TA system TA218433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6021112..6021707
• Replicon: chromosome
• Accession: NZ_CP084321
• Organism: Pseudomonas aeruginosa strain HS18-89

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LEL82_RS28240
• Protein ID: WP_003113526.1
• Coordinates: 6021429..6021707 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LEL82_RS28235
• Protein ID: WP_003099268.1
• Coordinates: 6021112..6021417 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEL82_RS28200 (LEL82_28155)	6016263..6017111	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
LEL82_RS28210 (LEL82_28165)	6017278..6018219	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
LEL82_RS28215 (LEL82_28170)	6018336..6018950	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
LEL82_RS28220 (LEL82_28175)	6018992..6019576	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
LEL82_RS28225 (LEL82_28180)	6019617..6020717	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
LEL82_RS28235 (LEL82_28190)	6021112..6021417	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
LEL82_RS28240 (LEL82_28195)	6021429..6021707	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LEL82_RS28245 (LEL82_28200)	6021760..6021888	-	129	Protein_5586	integrase	-
LEL82_RS28250 (LEL82_28205)	6022036..6024264	+	2229	WP_003099265.1	TonB-dependent receptor	-
LEL82_RS28255 (LEL82_28210)	6024334..6024981	-	648	WP_003095021.1	carbonate dehydratase	-
LEL82_RS28260 (LEL82_28215)	6025043..6026281	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T218433 WP_003113526.1 NZ_CP084321:c6021707-6021429 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T218433 NZ_CP115947:c812983-812729 [Ralstonia solanacearum MolK2]
ATGAGAGCCAAACACCAGAAGACGCTGGAACTGATTTTTTCCCGCCCTACGCCAGCGAGCGTTAAGTGGGCCGATGCCGT
CGCGCTCATGAAAGAACTGGGGGCAGAGTTGGAGGACCGCGAGGGTTCCCGCGTGGCCGTCTTCCTGTTTGGTCAAGTTA
AAGTGATGCATAGACCACACCCATCGCCCGATATAGACAAGGGCGCTGTGGCGTCGATACGCAAGTGGTTCGAGGAAAAC
GGAGTCAAGCCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT218433 WP_003099268.1 NZ_CP084321:c6021417-6021112 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT218433 NZ_CP115947:c812732-812394 [Ralstonia solanacearum MolK2]
ATGATCAACGTCATGAACATCGGCGGCCATAAGGCTGTCATCGCCTACGATCCAGATATCGAAATGTTCCGTGGGGAGTT
TGTTGGCCTGAACGGTGGCGCCGATTTCTACGCCGCCGATGTGCCCGGCCTGCACCGCGAAGGCGAGTTGTCGCTGCGTG
TGTTTTTGGACGAGTGCGCACGGCGTGGCGTTGAACCGCAAAAGCACTTTTCCGGCAGGTTCGTGCTGCGCGTGGAGGGC
AAGGTGCATGAGGCCGCCGCTATTGCCGCCGCCGCGCAAGGCGTGAGCCTTAACCAATGGGCAGCCGATGTGCTGGAGCA
AGCCGCCGAAGTAGCTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3