Detailed information of TA system TA218418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 22529..23114
• Replicon: plasmid pXMC5X702-tetX-145k
• Accession: NZ_CP084302
• Organism: Acinetobacter pseudolwoffii strain XMC5X702

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: LDO52_RS15335
• Protein ID: WP_000897307.1
• Coordinates: 22529..22849 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDO52_RS15340
• Protein ID: WP_000369781.1
• Coordinates: 22842..23114 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO52_RS15295 (LDO52_15295)	17884..18216	+	333	WP_117008139.1	MerR family DNA-binding protein	-
LDO52_RS15300 (LDO52_15300)	18463..18750	+	288	WP_000438825.1	BrnT family toxin	-
LDO52_RS15305 (LDO52_15305)	18737..19051	+	315	WP_004728120.1	BrnA antitoxin family protein	-
LDO52_RS15310 (LDO52_15310)	19140..19670	-	531	WP_000172759.1	hypothetical protein	-
LDO52_RS15315 (LDO52_15315)	19861..21186	+	1326	WP_001280601.1	ISNCY family transposase	-
LDO52_RS15320 (LDO52_15320)	21306..21608	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDO52_RS15325 (LDO52_15325)	21609..21890	+	282	WP_000985609.1	putative addiction module antidote protein	-
LDO52_RS15330 (LDO52_15330)	21970..22389	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
LDO52_RS15335 (LDO52_15335)	22529..22849	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDO52_RS15340 (LDO52_15340)	22842..23114	+	273	WP_000369781.1	NadS family protein	Antitoxin
LDO52_RS15345 (LDO52_15345)	23608..25083	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LDO52_RS15350 (LDO52_15350)	25139..26023	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
LDO52_RS15355 (LDO52_15355)	26261..27400	+	1140	Protein_30	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / floR / blaTEM-2 / aac(3)-IIa / aph(6)-Id / sul2 / dfrA36 / tet(X3) / tet(X6) / lnu(G)	-	1..145524	145524
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	15422..30732	15310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T218418 WP_000897307.1 NZ_CP084302:22529-22849 [Acinetobacter pseudolwoffii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T218418 NZ_CP115942:c66742-66437 [Ralstonia syzygii]
ATGATGATGGCCCAACGCACCATAAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTATCTCGACGCCTGCCTGGAGGAAGCCGGGGATGACCCCGCATTCATCACGCACGCGCTGGGCGTGGTCGCGCGGGCGC
GCGGCATGACGCAGATCGCCCGCGAGACCGGGATGACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT218418 WP_000369781.1 NZ_CP084302:22842-23114 [Acinetobacter pseudolwoffii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT218418 NZ_CP115942:c67047-66739 [Ralstonia syzygii]
ATGCTGACAATCCGCACGACTGAAATCTTCGACGACTGGTTTTACAGTCTCCGCGACAAGGCCGCGCAACGCCGCGTCCA
GGTGCGCATCGATCGCCTGCAGATGGGCAATCCAGGCGATGTGAAAACCGTACGTGAAGGCATCCGTGAGATGCGGATCG
ATCATGGCCCGGGCTATCGCGTGTATGTTGCGCAGCGTGGCGTGGTCCTGATCGTCCTGCTGTGCGGCGGTGACAAGTCG
ACGCAGGAAGCCGATGTCCGCCGCGCAATCGACCTGTCCCGCCGTCTTGACGTGGACAACATGGAATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3