Detailed information of TA system TA218414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 17992..18643
• Replicon: plasmid pXMC5X702-195k
• Accession: NZ_CP084301
• Organism: Acinetobacter pseudolwoffii strain XMC5X702

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: LDO52_RS14320
• Protein ID: WP_004668307.1
• Coordinates: 18287..18643 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDO52_RS14315
• Protein ID: WP_001140620.1
• Coordinates: 17992..18294 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO52_RS14285 (LDO52_14285)	13439..14779	-	1341	WP_225554359.1	ISNCY family transposase	-
LDO52_RS14290 (LDO52_14290)	15054..16334	-	1281	WP_004668302.1	cation transporter	-
LDO52_RS14295 (LDO52_14295)	16424..16816	+	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
LDO52_RS14300 (LDO52_14300)	16827..17312	-	486	WP_225554184.1	transposase	-
LDO52_RS14305 (LDO52_14305)	17360..17665	-	306	WP_087547287.1	IS630 transposase-related protein	-
LDO52_RS14310 (LDO52_14310)	17789..17950	+	162	Protein_13	IS6 family transposase	-
LDO52_RS14315 (LDO52_14315)	17992..18294	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
LDO52_RS14320 (LDO52_14320)	18287..18643	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDO52_RS14325 (LDO52_14325)	19330..19527	+	198	WP_100535484.1	hypothetical protein	-
LDO52_RS14330 (LDO52_14330)	19530..20960	-	1431	WP_100535483.1	PLP-dependent aminotransferase family protein	-
LDO52_RS14335 (LDO52_14335)	21054..21443	+	390	WP_225554361.1	hypothetical protein	-
LDO52_RS14340 (LDO52_14340)	21538..21828	-	291	WP_060875721.1	putative addiction module antidote protein	-
LDO52_RS14345 (LDO52_14345)	21830..22126	-	297	WP_005175534.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDO52_RS14350 (LDO52_14350)	22341..23114	-	774	WP_225554389.1	glucose 1-dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..195429	195429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T218414 WP_004668307.1 NZ_CP084301:c18643-18287 [Acinetobacter pseudolwoffii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T218414 NZ_CP115941:c1767524-1767237 [Ralstonia syzygii]
ATGACTGAAACCTACGCACCGTTTGACGCGGCGGCCTACCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCGGCCGC
GATGGAAGACCCGAATCCGGATGTCTTCCTGGCTGCCCTCGGTGCCGTTGCACGCGCCTGCGGCATTGCCAAGGTGGCTG
CGGACGCGGGCCTGGGGCGCGAAAGCCTGTACAAGGCCATCAGCCCCGGCGCGCACCCGCGCTACGAAACCATCCAGAAA
ATCATGCGCGCACTGGGCGTCAGGCTGAACGTGACGCCTGCCGCCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT218414 WP_001140620.1 NZ_CP084301:c18294-17992 [Acinetobacter pseudolwoffii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT218414 NZ_CP115941:c1767777-1767517 [Ralstonia syzygii]
CTGCGCGACAAGCGTGCCAAGGCGCGGATCGCCGCCCGGCTGGTCAGCGCATCGTTCGGGAATTTCGGCGACTGCAAGCC
GGTGGGTGAAGGCGTGAGCGAGATGCGGATCGATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACTGTCG
TCTACATCCTGCTCACGGGCGGCAACAAGGCAACCCAAGCCCAGGACATCAAGCAAGCCATCCGGATGGCACGCGACCTG
AAGGAGTCAGGCCATGACTGA

Structures

Toxin

• AlphaFold DB: N9NM16