Detailed information of TA system TA218399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4506496..4507159
• Replicon: chromosome
• Accession: NZ_CP084290
• Organism: Citrobacter werkmanii strain LHC5-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LD024_RS21420
• Protein ID: WP_227410043.1
• Coordinates: 4506857..4507159 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2Z3X2H4
• Locus tag: LD024_RS21415
• Protein ID: WP_038636361.1
• Coordinates: 4506496..4506837 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD024_RS21385 (4502020)	4502020..4502778	+	759	WP_038636380.1	phosphonate C-P lyase system protein PhnK	-
LD024_RS21390 (4502886)	4502886..4503566	+	681	WP_150344518.1	phosphonate C-P lyase system protein PhnL	-
LD024_RS21395 (4503563)	4503563..4504699	+	1137	WP_038636374.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
LD024_RS21400 (4504702)	4504702..4505256	+	555	WP_137347414.1	ribose 1,5-bisphosphokinase	-
LD024_RS21405 (4505243)	4505243..4505677	+	435	WP_069325128.1	aminoalkylphosphonate N-acetyltransferase	-
LD024_RS21410 (4505686)	4505686..4506444	+	759	WP_227410042.1	phosphonate metabolism protein PhnP	-
LD024_RS21415 (4506496)	4506496..4506837	-	342	WP_038636361.1	HigA family addiction module antitoxin	Antitoxin
LD024_RS21420 (4506857)	4506857..4507159	-	303	WP_227410043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LD024_RS21425 (4507315)	4507315..4507644	-	330	WP_069325126.1	DDRRRQL repeat protein YjdP	-
LD024_RS21430 (4507714)	4507714..4509978	-	2265	WP_150344515.1	hybrid sensor histidine kinase/response regulator	-
LD024_RS21435 (4510093)	4510093..4511616	+	1524	WP_038636350.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11890.51 Da
• Isoelectric Point: 9.9581

>T218399 WP_227410043.1 NZ_CP084290:c4507159-4506857 [Citrobacter werkmanii]
MSKSLNIRNFRDTWFEDFFERATPHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T218399 NZ_CP115930:325736-326104 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13063.93 Da
• Isoelectric Point: 7.2038

>AT218399 WP_038636361.1 NZ_CP084290:c4506837-4506496 [Citrobacter werkmanii]
MKQATRKPTTVGDVLQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIMSVKDFVAQRNVATKKVA

Nucleotide

• Length: 342 bp

>AT218399 NZ_CP115930:325518-325739 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2Z3X2H4