Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 230995..231530 Replicon plasmid pY3-1
Accession NZ_CP084247
Organism Enterococcus lactis strain DH9003

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag K9N64_RS02045 Protein ID WP_002314366.1
Coordinates 230995..231267 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag K9N64_RS02050 Protein ID WP_002297422.1
Coordinates 231267..231530 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9N64_RS02015 (K9N64_02010) 226001..227188 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
K9N64_RS02020 (K9N64_02015) 227376..228323 + 948 WP_002303155.1 sugar-binding domain-containing protein -
K9N64_RS02025 (K9N64_02020) 228620..229168 - 549 WP_002292681.1 hypothetical protein -
K9N64_RS02030 (K9N64_02025) 229169..230026 - 858 WP_002292680.1 ParA family protein -
K9N64_RS02035 (K9N64_02030) 230312..230599 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
K9N64_RS02040 (K9N64_02035) 230589..230918 - 330 WP_002292678.1 AbrB family transcriptional regulator -
K9N64_RS02045 (K9N64_02040) 230995..231267 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
K9N64_RS02050 (K9N64_02045) 231267..231530 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K9N64_RS02055 (K9N64_02050) 231636..232562 - 927 WP_016177567.1 hypothetical protein -
K9N64_RS02060 (K9N64_02055) 233213..233857 - 645 WP_002289351.1 IS6 family transposase -
K9N64_RS02065 (K9N64_02060) 234386..235873 - 1488 WP_002290546.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..292841 292841
- flank IS/Tn - - 233213..233857 644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T218367 WP_002314366.1 NZ_CP084247:c231267-230995 [Enterococcus lactis]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T218367 NZ_CP115887:1894704-1894799 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT218367 WP_002297422.1 NZ_CP084247:c231530-231267 [Enterococcus lactis]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT218367 NZ_CP115887:c1894884-1894827 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References