Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3818485..3819082 Replicon chromosome
Accession NZ_CP084241
Organism Klebsiella pneumoniae strain T877

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LDH71_RS18595 Protein ID WP_004142563.1
Coordinates 3818765..3819082 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LDH71_RS18590 Protein ID WP_004142561.1
Coordinates 3818485..3818772 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDH71_RS18560 (3814566) 3814566..3814814 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LDH71_RS18565 (3814832) 3814832..3815173 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LDH71_RS18570 (3815204) 3815204..3816319 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
LDH71_RS18575 (3816498) 3816498..3817079 + 582 WP_217348155.1 TetR/AcrR family transcriptional regulator -
LDH71_RS18580 (3817079) 3817079..3817447 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LDH71_RS18585 (3817567) 3817567..3818220 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LDH71_RS18590 (3818485) 3818485..3818772 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LDH71_RS18595 (3818765) 3818765..3819082 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LDH71_RS18600 (3819267) 3819267..3820310 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
LDH71_RS18605 (3820977) 3820977..3821843 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
LDH71_RS18610 (3821952) 3821952..3823379 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T218359 WP_004142563.1 NZ_CP084241:c3819082-3818765 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T218359 NZ_CP115874:3455655-3455978 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCGCCCGTACAGGTCTGGCAGAAACTGTTAAC
GTATCTGCTGGAGCACCACTACGGCCTGACACTCAACGACACACCATTTCATGACGACACGGCCATTGAGGAGCATATCG
ATGCGGGAATAACGCTTGCCGATGCTGTTAATTTCCTGGTCGAAAAGTACGAACTGGCTCGTATCGACCGCAAGGGATTT
ACATGGCGAGAGCAACCCCCCTTCATTACTGCAACGGATATTCTGCGTGCCAAAAAGAACATCGGGCTGTTGCCCAGATC
TTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT218359 WP_004142561.1 NZ_CP084241:c3818772-3818485 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT218359 NZ_CP115874:3455300-3455623 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCAAAATTCATGGGGACTGAAGCGCGACATCTCGCCGTGCTTCGGTGCAAGGCTTGTGCAGGAAGGCTGTCGCCTGCA
TTTTCTGGCAAACCGTGCAGGTATTAGCGGTGCGTTTAACGAGGAGGAGGCGCTACGTCTGGATCAGGCATTTCCGCTAG
TGATGAAACAGCTTGAATTGATGCTGACAACCGGTGAACTGAATCCGCGCCATCAGCATTGCGTCACGTTGCATCATAAC
GGACTAACTTGTGAAGCTGACACTCTCGGCTCTCATGGCTATGTTTACATCGTTATTTATCCCCAAGCATCTAAAACCAG
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References