Detailed information of TA system TA218326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1443519..1444123
• Replicon: chromosome
• Accession: NZ_CP084222
• Organism: Arsenophonus apicola strain ArsBeeUS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDL57_RS06850
• Protein ID: WP_180560067.1
• Coordinates: 1443519..1443830 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDL57_RS06855
• Protein ID: WP_180560066.1
• Coordinates: 1443833..1444123 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL57_RS06825 (LDL57_06825)	1438657..1438821	+	165	WP_180558904.1	hypothetical protein	-
LDL57_RS06830 (LDL57_06830)	1439130..1439435	+	306	WP_225507354.1	DUF1902 domain-containing protein	-
LDL57_RS06835 (LDL57_06835)	1439458..1439748	+	291	WP_180558906.1	type II toxin-antitoxin system HicA family toxin	-
LDL57_RS06840 (LDL57_06840)	1439893..1442778	+	2886	WP_225507356.1	phage tail tape measure protein	-
LDL57_RS06845 (LDL57_06845)	1442882..1443394	+	513	WP_180560068.1	zinc ribbon domain-containing protein	-
LDL57_RS06850 (LDL57_06850)	1443519..1443830	+	312	WP_180560067.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDL57_RS06855 (LDL57_06855)	1443833..1444123	+	291	WP_180560066.1	NadS family protein	Antitoxin
LDL57_RS06860 (LDL57_06860)	1444301..1444555	+	255	WP_180560065.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LDL57_RS06865 (LDL57_06865)	1444545..1444838	+	294	WP_180560064.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDL57_RS06870 (LDL57_06870)	1445077..1445424	+	348	WP_225507358.1	phage tail protein	-
LDL57_RS06875 (LDL57_06875)	1445421..1446164	+	744	WP_180560063.1	phage minor tail protein L	-
LDL57_RS06880 (LDL57_06880)	1446161..1446874	+	714	WP_180560062.1	C40 family peptidase	-
LDL57_RS06885 (LDL57_06885)	1446871..1447467	+	597	WP_180560061.1	tail assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1419238..1471118	51880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11905.66 Da
• Isoelectric Point: 8.8372

>T218326 WP_180560067.1 NZ_CP084222:1443519-1443830 [Arsenophonus apicola]
MIFIETVIFTEDCKGLLTDDEYREFQQYLADNPDAGDVIQRTGGLRKVRWASSGKGKRAGVRVIYYHKVDVSHIRLLLIY
KKGIKDDLSESEKKVLRTLNEGW

Nucleotide

• Length: 312 bp

>T218326 NZ_CP115833:c32388-32101 [Klebsiella pneumoniae]
ATGACATATACCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGAACAAGCTAGATAAGACTATTCAGCAGCAGTTTGC
AAAGAAACTTAAAAAGTGCTGTGAAAACCCACACATTCCTTCAGCAAAACTACGTGGTATAAAGGATTGCTATAAAATAA
AACTGCGAGCATCTGGTTTTCGCCTCGTCTATCAGGTCATAGATAATCAACTTATAATTGCTATTGTGGCTGTAGGCAAA
CGGGAGCGCAGTGACGTTTATACACTGGCGAGTGAACGTATGAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10673.46 Da
• Isoelectric Point: 10.8619

>AT218326 WP_180560066.1 NZ_CP084222:1443833-1444123 [Arsenophonus apicola]
MDKKLFSRLTESMEQMNEIISGERAPSRETMVEAVKVKQIRKATGLSQAGFAKLISVNVGTLRNWEQGRRDPTGPAKALL
RAIEKDPVHVLKALSI

Nucleotide

• Length: 291 bp

>AT218326 NZ_CP115833:c32636-32385 [Klebsiella pneumoniae]
ATGCCACATATCATTCTGAGTGACACGACTGCCAGCGTCAGTGAATTGAAAAAAAACCCGATGGCCACAGTCAGCGCAGG
TGACGGGTTCCCAGTAGCGATTCTTAACCGGAACCAGCCAGCTTTCTATTGCGTGCCTGCGGAACTCTACGAAAAGATGC
TTGATGCGCTGGATGATCAGGAACTCATTAAACTGGTTGCAGAACGCAGTAATCAACCGCTGTTTGATATGGACCTGGAG
AAGTACCTATGA