Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 961488..962154 Replicon chromosome
Accession NZ_CP084222
Organism Arsenophonus apicola strain ArsBeeUS

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LDL57_RS04220 Protein ID WP_180560260.1
Coordinates 961831..962154 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LDL57_RS04215 Protein ID WP_180560259.1
Coordinates 961488..961784 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL57_RS04200 (LDL57_04200) 957916..958335 + 420 WP_225507127.1 tail fiber assembly protein -
LDL57_RS04205 (LDL57_04205) 959037..959603 + 567 WP_225507469.1 hypothetical protein -
LDL57_RS04210 (LDL57_04210) 959606..961282 + 1677 WP_225507128.1 phage baseplate assembly protein V -
LDL57_RS04215 (LDL57_04215) 961488..961784 - 297 WP_180560259.1 NadS family protein Antitoxin
LDL57_RS04220 (LDL57_04220) 961831..962154 - 324 WP_180560260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LDL57_RS04225 (LDL57_04225) 962557..962856 + 300 Protein_808 BRO family protein -
LDL57_RS04230 (LDL57_04230) 963643..964014 - 372 WP_180560262.1 hypothetical protein -
LDL57_RS04235 (LDL57_04235) 964531..964857 + 327 Protein_810 BRO family protein -
LDL57_RS04245 (LDL57_04245) 965647..966129 - 483 WP_180560264.1 SsrA-binding protein SmpB -
LDL57_RS04250 (LDL57_04250) 966287..966721 + 435 WP_026821677.1 type II toxin-antitoxin system RatA family toxin -
LDL57_RS04255 (LDL57_04255) 966714..967004 + 291 WP_180560266.1 RnfH family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - rfaE 899538..965133 65595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(34-104)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-92)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12558.73 Da        Isoelectric Point: 9.9388

>T218324 WP_180560260.1 NZ_CP084222:c962154-961831 [Arsenophonus apicola]
MEYLLFIETPVFSRERTELLTDDEFRQLQQYLLKNHEQGSTISATGGCKKIRWLREGMGKQGGVRIIYYTMTKIGKIYLL
LIYPKNKKDDLTSKEKEVLKAIINKLQ

Download         Length: 324 bp

>T218324 NZ_CP115832:c89369-88887 [Klebsiella pneumoniae]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACACAACATTGCTGAGTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAATCACAGAACGGGCATTTCAAGAGTTTTCGTCGTCTGTGCTGAAAACACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCTGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTAGGAGCTGCGCTGCT
TAAGGATGCGATATACCGAACAGAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCACTGACATTATTATTTCCGATCAAAACA
TGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.90 Da        Isoelectric Point: 10.0123

>AT218324 WP_180560259.1 NZ_CP084222:c961784-961488 [Arsenophonus apicola]
MDKKLFGDLVESMNQMVAMEKGERSIPSENIHRHRLPNVKNLRETFGLKQSEFAEAVGVSASLIQSWEQHRRIPSGSALK
LLKMLERNPTLINELSTL

Download         Length: 297 bp

>AT218324 NZ_CP115832:c89638-89360 [Klebsiella pneumoniae]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAATCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAGCCGCAAATCGCCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References