Detailed information of TA system TA218299

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101649..101902
• Replicon: plasmid pKP2648-KPCR
• Accession: NZ_CP084211
• Organism: Escherichia coli strain EC2648-R2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LDB16_RS23235
• Protein ID: WP_001312851.1
• Coordinates: 101753..101902 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	101649..101708 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB16_RS23195 (96682)	96682..96987	+	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-
LDB16_RS23200 (97007)	97007..97372	+	366	Protein_120	type IV conjugative transfer system protein TraE	-
LDB16_RS23205 (97427)	97427..98131	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LDB16_RS23210 (98156)	98156..98356	+	201	WP_072354025.1	hypothetical protein	-
LDB16_RS23215 (98376)	98376..99122	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LDB16_RS23220 (99177)	99177..99737	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LDB16_RS23225 (99869)	99869..100069	+	201	WP_015059022.1	hypothetical protein	-
LDB16_RS23230 (100455)	100455..101054	+	600	WP_032083981.1	PIN domain-containing protein	-
- (101649)	101649..101708	-	60	NuclAT_0	-	Antitoxin
- (101649)	101649..101708	-	60	NuclAT_0	-	Antitoxin
- (101649)	101649..101708	-	60	NuclAT_0	-	Antitoxin
- (101649)	101649..101708	-	60	NuclAT_0	-	Antitoxin
LDB16_RS23235 (101753)	101753..101902	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LDB16_RS23240 (102186)	102186..102434	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaSHV-187 / blaKPC-2	-	1..102745	102745
	-	flank	IS/Tn	-	-	97427..98131	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T218299 WP_001312851.1 NZ_CP084211:101753-101902 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T218299 NZ_CP115812:1093721-1093987 [Enterococcus faecium]
ATGACCTACCGTTTAGAATTCACGCCTGAAATTCAGAAACAACTTCGTAAAATGGACAAGCATCAAGCGACGCTGATTGT
TCGATGGCTGTATCAACATATTGATGGTATTGACGATCCGAGAAAATTCGGAAAAGGATTGACCGCTAATCGTTCAGGAC
AATGGCGTTATCGAATTGGCAAATATCGTGTGTTGGTAGAGATAGAAGATGATCAAATGGTTGTTATCGCTATTCAAGTG
GGACACCGAAGAAATATCTATGATTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT218299 NZ_CP084211:c101708-101649 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin