Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3594747..3595288 Replicon chromosome
Accession NZ_CP084209
Organism Escherichia coli strain EC2648-R2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LDB16_RS17595 Protein ID WP_000615983.1
Coordinates 3595010..3595288 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LDB16_RS17590 Protein ID WP_000729703.1
Coordinates 3594747..3595007 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB16_RS17570 (3590422) 3590422..3591207 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LDB16_RS17575 (3591179) 3591179..3592891 + 1713 Protein_3455 flagellar biosynthesis protein FlhA -
LDB16_RS17580 (3593115) 3593115..3593612 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LDB16_RS17585 (3593788) 3593788..3594537 - 750 WP_000056849.1 C40 family peptidase -
LDB16_RS17590 (3594747) 3594747..3595007 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LDB16_RS17595 (3595010) 3595010..3595288 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LDB16_RS17600 (3595444) 3595444..3596184 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LDB16_RS17605 (3596155) 3596155..3596922 - 768 WP_000333380.1 class II glutamine amidotransferase -
LDB16_RS17610 (3597128) 3597128..3597706 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T218292 WP_000615983.1 NZ_CP084209:3595010-3595288 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T218292 NZ_CP115780:c88416-88153 [Paracoccus aerodenitrificans]
ATGGATTTTGAGTATGACCCCGCCAAAAGTGCCTCGAATCTGTCGAAGCATGGCATCGACTTCGATCAGGCACAGGCTCT
TTGGGATGACCCGTGGATGCTCGAAGCGCCCGCCAAGACCGAGGATGAACCGCGGCTCATCTCGATTGGGAAGATCGAGG
GCAAGCACTGGGCAGCCGTCTGGACGCCGCGAGGTAATGCGGTGCGGATCATCTCGGTGCGCCGCGCCCGAAAAGAGGAG
ATCAGCTACTATGAAGGCAACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT218292 WP_000729703.1 NZ_CP084209:3594747-3595007 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT218292 NZ_CP115780:c88166-87951 [Paracoccus aerodenitrificans]
ATGAAGGCAACTGAGTTCGATAAGAAATTCGACACCGATGAGAACGTGAGCGATGCAATCGACTGGTCCCAGGCGCGGCG
TCCGAACGACCAACCCAAGCGCGTGAACGTGGATTTTCCGGCGTGGGTCGTCGAGGGCCTCGACAAGCAGGCTCGGCACT
TGGGCGTGACGCGGCAATCGCTAATCAAGCTATGGATTGCCGAACGGCTCCAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References