Detailed information of TA system TA021829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3543106..3543711
• Replicon: chromosome
• Accession: NC_008740
• Organism: Marinobacter aquaeolei VT8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Z1BUV4
• Locus tag: MAQU_RS16325
• Protein ID: WP_011786639.1
• Coordinates: 3543433..3543711 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MAQU_RS16320
• Protein ID: WP_095209522.1
• Coordinates: 3543106..3543405 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAQU_RS16290	3538253..3539182	-	930	WP_011786632.1	chromate resistance protein	-
MAQU_RS16295	3539354..3540283	+	930	WP_011786633.1	CDF family Co(II)/Ni(II) efflux transporter DmeF	-
MAQU_RS16300	3540315..3540593	-	279	WP_011786634.1	metal-sensing transcriptional repressor	-
MAQU_RS16305	3540674..3541057	+	384	WP_041656491.1	hypothetical protein	-
MAQU_RS16310	3541106..3541840	+	735	WP_011786636.1	HupE/UreJ family protein	-
MAQU_RS16315	3541856..3542557	+	702	WP_011786637.1	hypothetical protein	-
MAQU_RS16320	3543106..3543405	-	300	WP_095209522.1	HigA family addiction module antidote protein	Antitoxin
MAQU_RS16325	3543433..3543711	-	279	WP_011786639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAQU_RS16330	3544405..3544884	-	480	WP_011786640.1	YqhA family protein	-
MAQU_RS16335	3544937..3546184	-	1248	WP_011785114.1	IS256 family transposase	-
MAQU_RS16340	3546568..3546981	-	414	WP_041656492.1	immunity 70 family protein	-
MAQU_RS16345	3547114..3547398	-	285	WP_011786641.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3544937..3546184	1247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10765.21 Da
• Isoelectric Point: 8.5359

>T21829 WP_011786639.1 NC_008740:c3543711-3543433 [Marinobacter hydrocarbonoclasticus VT8]
MIKSFKCKHTEELVKTGKTRRFNAIQRTAERKLQQLLSAEALKDLKSPPGNRLEPLSGDREGQHSIRINDQWRLCFRWTD
EGAEDVEIVDYH

Nucleotide

• Length: 279 bp

>T21829 NC_008740:c3543711-3543433 [Marinobacter hydrocarbonoclasticus VT8]
ATGATCAAGAGCTTTAAGTGCAAGCACACAGAGGAGCTTGTAAAAACAGGTAAGACTCGGCGTTTCAATGCAATCCAGCG
AACGGCCGAGCGTAAGTTACAGCAGCTGCTAAGCGCTGAAGCTCTTAAAGATTTAAAGTCTCCGCCCGGGAATAGACTTG
AGCCGCTTTCTGGAGACAGAGAGGGGCAACACAGCATCCGGATCAATGACCAGTGGCGATTGTGTTTCCGCTGGACAGAT
GAAGGGGCGGAAGACGTGGAGATTGTTGATTACCACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11569.29 Da
• Isoelectric Point: 9.4576

>AT21829 WP_095209522.1 NC_008740:c3543405-3543106 [Marinobacter hydrocarbonoclasticus VT8]
MRPIHPGEILLEDYLEPLGMSVNALSKELYVPAQRLNEIVRERRGVTADTAMRLARYFGTTEQFWLNLQTDYDLRRVRAE
KSDQIKKLIKPRKSDHEAA

Nucleotide

• Length: 300 bp

>AT21829 NC_008740:c3543405-3543106 [Marinobacter hydrocarbonoclasticus VT8]
ATGCGCCCTATCCATCCAGGAGAGATTTTACTTGAGGATTACCTTGAGCCTCTTGGCATGAGTGTGAATGCGCTTTCAAA
AGAACTTTATGTTCCTGCACAGCGCCTGAACGAGATTGTTCGCGAGCGTCGTGGTGTGACCGCGGATACGGCCATGCGTT
TGGCTCGTTACTTTGGGACTACTGAGCAGTTTTGGCTCAATTTGCAGACCGATTATGACCTGCGTCGTGTCCGCGCGGAG
AAATCCGATCAAATCAAGAAGCTCATCAAGCCTCGAAAGTCTGATCATGAGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A4Z1BUV4