Detailed information of TA system TA218276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1790537..1791039
• Replicon: chromosome
• Accession: NZ_CP084209
• Organism: Escherichia coli strain EC2648-R2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: LDB16_RS08500
• Protein ID: WP_000767829.1
• Coordinates: 1790785..1791039 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LDB16_RS08495
• Protein ID: WP_001259255.1
• Coordinates: 1790537..1790788 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB16_RS08470 (1785715)	1785715..1786782	-	1068	WP_000080105.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
LDB16_RS08475 (1786782)	1786782..1787852	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
LDB16_RS08480 (1787849)	1787849..1789153	-	1305	WP_000009594.1	histidinol dehydrogenase	-
LDB16_RS08485 (1789159)	1789159..1790058	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LDB16_RS08490 (1790204)	1790204..1790254	-	51	WP_001364200.1	his operon leader peptide	-
LDB16_RS08495 (1790537)	1790537..1790788	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LDB16_RS08500 (1790785)	1790785..1791039	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LDB16_RS08505 (1791122)	1791122..1791946	+	825	WP_000754737.1	SDR family oxidoreductase	-
LDB16_RS08510 (1791992)	1791992..1792921	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
LDB16_RS08515 (1793136)	1793136..1793198	+	63	WP_010723108.1	membrane protein YoeI	-
LDB16_RS08520 (1793188)	1793188..1794546	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
LDB16_RS08525 (1794725)	1794725..1795783	+	1059	WP_000492339.1	thiosulfate utilization transporter TsuA/YeeE	-
LDB16_RS08530 (1795797)	1795797..1796024	+	228	WP_000234896.1	sulfurtransferase TusA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T218276 WP_000767829.1 NZ_CP084209:1790785-1791039 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T218276 NZ_CP115716:c122425-122120 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT218276 WP_001259255.1 NZ_CP084209:1790537-1790788 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT218276 NZ_CP115716:c122645-122427 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0