Detailed information of TA system TA218262

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19951..20204
• Replicon: plasmid pKPC-fusion-1
• Accession: NZ_CP084208
• Organism: Escherichia coli strain EC2648-R1-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LDB15_RS22490
• Protein ID: WP_001312851.1
• Coordinates: 20055..20204 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	19951..20010 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB15_RS22450 (14984)	14984..15289	+	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-
LDB15_RS22455 (15309)	15309..15674	+	366	Protein_25	type IV conjugative transfer system protein TraE	-
LDB15_RS22460 (15729)	15729..16433	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LDB15_RS22465 (16458)	16458..16658	+	201	WP_072354025.1	hypothetical protein	-
LDB15_RS22470 (16678)	16678..17424	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LDB15_RS22475 (17479)	17479..18039	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LDB15_RS22480 (18171)	18171..18371	+	201	WP_015059022.1	hypothetical protein	-
LDB15_RS22485 (18757)	18757..19356	+	600	WP_032083981.1	PIN domain-containing protein	-
- (19951)	19951..20010	-	60	NuclAT_0	-	Antitoxin
- (19951)	19951..20010	-	60	NuclAT_0	-	Antitoxin
- (19951)	19951..20010	-	60	NuclAT_0	-	Antitoxin
- (19951)	19951..20010	-	60	NuclAT_0	-	Antitoxin
LDB15_RS22490 (20055)	20055..20204	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LDB15_RS22495 (20488)	20488..20736	+	249	WP_000083837.1	replication regulatory protein RepA	-
LDB15_RS23395 (20981)	20981..21055	+	75	WP_001375168.1	RepA leader peptide Tap	-
LDB15_RS22505 (21048)	21048..21905	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
LDB15_RS22510 (22844)	22844..23497	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LDB15_RS22515 (23590)	23590..23847	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LDB15_RS22520 (23780)	23780..24181	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LDB15_RS22525 (24430)	24430..24845	+	416	Protein_39	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaSHV-187 / blaKPC-2	-	1..139530	139530
	-	flank	IS/Tn	-	-	15729..16433	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T218262 WP_001312851.1 NZ_CP084208:20055-20204 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T218262 NZ_CP115713:c3982877-3982473 [Rhodopseudomonas palustris]
GTGAGGGCGTTCTTCGATACCAATATTTTGGTCTACTCCACGACGTCGGACCCGCGGCAAGCAACGGCCGCCGCTTGTCT
GGAGCAGGGCGGGTTTGCCAGCGTTCAGGTGCTCAACGAATTCGTCCACGTGGCGCGCCGCAAGCTGCGGCACGACTGGC
CGCAGATCGAAGTCGCGCTCGAGCAGTTTCACACCGCGCTCGATGACGTACTTCCGATCACTCTAACCACGCATGCCGCA
GCCGTTGTCCTTGCACGCGATCACCGGGTGTCGTTCTACGATGCGCTGATCGTCGCAGCGGCCCAGGAGGCTGGCTGCGA
TGTGCTGTACAGCGAAGACTTGCAGCACGGCCGCAGCTTCGGCACCTTGCGCGTCGAGAATCCATTTCTGGAAGGCTCGC
GATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT218262 NZ_CP084208:c20010-19951 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin