Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4403874..4404415 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LDB15_RS21410 Protein ID WP_000615983.1
Coordinates 4404137..4404415 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LDB15_RS21405 Protein ID WP_000729703.1
Coordinates 4403874..4404134 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS21385 (4399549) 4399549..4400334 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LDB15_RS21390 (4400306) 4400306..4402018 + 1713 Protein_4188 flagellar biosynthesis protein FlhA -
LDB15_RS21395 (4402242) 4402242..4402739 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LDB15_RS21400 (4402915) 4402915..4403664 - 750 WP_000056849.1 C40 family peptidase -
LDB15_RS21405 (4403874) 4403874..4404134 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LDB15_RS21410 (4404137) 4404137..4404415 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LDB15_RS21415 (4404571) 4404571..4405311 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LDB15_RS21420 (4405282) 4405282..4406049 - 768 WP_000333380.1 class II glutamine amidotransferase -
LDB15_RS21425 (4406255) 4406255..4406833 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T218261 WP_000615983.1 NZ_CP084207:4404137-4404415 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T218261 NZ_CP115713:3658009-3658293 [Rhodopseudomonas palustris]
ATGATCCAGGGCTTTCGAGGCAAGTTTGCTCGGGCGATTTTGCATGACCGGAAAGCCCCCAAGGGCCTTCCGGCCGACCT
TCTCGGCACTGCCCGGCGCAAACTGGTTCAGCTCAACGCCGCCGCTGCTCTGGCCGACCTTGCGATCCCTCCCGGCAATC
GCCTGGAAGCCCTACGCGGAGATTTACAGGGTCTCCATTCCATCCGGATCAACGATCAGTGGCGGATCGTGTTTCGCTGG
AAAGACACTGGTCCGGAAGACGTCGAGATCGTCGACTATCACTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT218261 WP_000729703.1 NZ_CP084207:4403874-4404134 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT218261 NZ_CP115713:3658306-3658608 [Rhodopseudomonas palustris]
ATGACGAAGAAACTCCCCCCTGTTCATCCCGGCGAAATTCTCCGCGAAGAGTTTCTGCTGCCGATGAACCTGTCGCCCTA
CGCCGTCGCCGCAGCCTGCGGCGTACCGCGCACGCGCATCGAACGGTTGGCACGGCAGGAAACCCCAGTAACGGCGGACA
CCGCGCTGCGGCTGGCGAGATATTTCGGCACGACGCCGAATTTCTGGATGAACATGCAGGCGCAGTACGATCTGGAGGTC
GCGGAAGACAACAGCGCCGACGAACTCAGGCGGATCAAGCCCATCAAGTCGAAAGCGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References