Detailed information of TA system TA218260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4397677..4398371
• Replicon: chromosome
• Accession: NZ_CP084207
• Organism: Escherichia coli strain EC2648-R1-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LDB15_RS21370
• Protein ID: WP_001263489.1
• Coordinates: 4397677..4398075 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LDB15_RS21375
• Protein ID: WP_000554758.1
• Coordinates: 4398078..4398371 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4393265)	4393265..4393345	-	81	NuclAT_12	-	-
- (4393265)	4393265..4393345	-	81	NuclAT_12	-	-
- (4393265)	4393265..4393345	-	81	NuclAT_12	-	-
- (4393265)	4393265..4393345	-	81	NuclAT_12	-	-
LDB15_RS21345 (4393941)	4393941..4394399	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LDB15_RS21350 (4394660)	4394660..4396117	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LDB15_RS21355 (4396174)	4396174..4396695	-	522	Protein_4181	peptide chain release factor H	-
LDB15_RS21360 (4396691)	4396691..4396897	-	207	Protein_4182	RtcB family protein	-
LDB15_RS21365 (4397215)	4397215..4397667	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LDB15_RS21370 (4397677)	4397677..4398075	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LDB15_RS21375 (4398078)	4398078..4398371	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LDB15_RS21380 (4398423)	4398423..4399478	-	1056	WP_001226164.1	DNA polymerase IV	-
LDB15_RS21385 (4399549)	4399549..4400334	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LDB15_RS21390 (4400306)	4400306..4402018	+	1713	Protein_4188	flagellar biosynthesis protein FlhA	-
LDB15_RS21395 (4402242)	4402242..4402739	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T218260 WP_001263489.1 NZ_CP084207:c4398075-4397677 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T218260 NZ_CP115713:2904283-2904570 [Rhodopseudomonas palustris]
ATGATCCGGTCATGGCGCAATGCTGCCACCCGGAAGTTATGGGACGGCAGCCGGTTCGACCAATTCCGCGGCCTTGATGT
CGAGGCCGCGATCGATCTGTTGCTGGCTTTGAATGCCGCCAAGTCGCTGCAAGATCTCAGTCCTCTACAGAGCGTAGGAT
TACACAAACTCAAGGGCGTGCGCAGGAACCAATGGGCGATGACGGTCAACGACCGGTGGCGGATTTGTTTCGAGTTTCGC
AAAGGTGATGCGTTCGAAGTCGAGATCGTCGACTATCACAAAGGGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT218260 WP_000554758.1 NZ_CP084207:c4398371-4398078 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT218260 NZ_CP115713:2904584-2904871 [Rhodopseudomonas palustris]
ATGGCTCCTGTTTCCCATCCCGGCCGGCTGCTGAAGCGAGAACTGGCGGCGCGGAAACTCAGCGCCAACCGGCTGGCGCT
GGATATCGGCGTTCCGTCTGGACGGATCACCGACATTCTCAACGGTCGTCGGTCGATTACTGCGGATACCGCGGTGCGGC
TGGGTCGTTATTTCGGAAATGCTCCGCAGTTCTGGCTCGATCTGCAGAGCCAGTACGACATCGCCGTCATCGAGCGTGAC
AAGGGCGCGGAGATCGACCGTCAGGTGCGGCCGGCGGACGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3