Detailed information of TA system TA218231

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 110961..111373
• Replicon: chromosome
• Accession: NZ_CP084207
• Organism: Escherichia coli strain EC2648-R1-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LDB15_RS00525
• Protein ID: WP_000132601.1
• Coordinates: 111032..111373 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	110961..111037 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB15_RS00515 (107824)	107824..109413	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
LDB15_RS00520 (109410)	109410..110804	+	1395	WP_072647344.1	type I restriction-modification system specificity subunit	-
- (110961)	110961..111037	-	77	NuclAT_14	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_14	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_14	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_14	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_15	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_15	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_15	-	Antitoxin
- (110961)	110961..111037	-	77	NuclAT_15	-	Antitoxin
LDB15_RS00525 (111032)	111032..111373	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
LDB15_RS00530 (111535)	111535..112914	+	1380	WP_072647343.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LDB15_RS00535 (112914)	112914..113960	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T218231 WP_000132601.1 NZ_CP084207:111032-111373 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T218231 NZ_CP115707:814049-814459 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT218231 NZ_CP084207:c111037-110961 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin