Detailed information of TA system TA218226

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70589..70842
• Replicon: plasmid pKPC-fusion
• Accession: NZ_CP084206
• Organism: Escherichia coli strain EC2648-R1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LDB14_RS22735
• Protein ID: WP_001312851.1
• Coordinates: 70589..70738 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	70783..70842 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB14_RS22700 (65948)	65948..66363	-	416	Protein_74	IS1-like element IS1B family transposase	-
LDB14_RS22705 (66612)	66612..67013	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LDB14_RS22710 (66946)	66946..67203	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LDB14_RS22715 (67296)	67296..67949	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LDB14_RS22720 (68888)	68888..69745	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
LDB14_RS23385 (69738)	69738..69812	-	75	WP_001375168.1	RepA leader peptide Tap	-
LDB14_RS22730 (70057)	70057..70305	-	249	WP_000083837.1	replication regulatory protein RepA	-
LDB14_RS22735 (70589)	70589..70738	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (70783)	70783..70842	+	60	NuclAT_0	-	Antitoxin
- (70783)	70783..70842	+	60	NuclAT_0	-	Antitoxin
- (70783)	70783..70842	+	60	NuclAT_0	-	Antitoxin
- (70783)	70783..70842	+	60	NuclAT_0	-	Antitoxin
LDB14_RS22740 (71437)	71437..72036	-	600	WP_032083981.1	PIN domain-containing protein	-
LDB14_RS22745 (72422)	72422..72622	-	201	WP_015059022.1	hypothetical protein	-
LDB14_RS22750 (72754)	72754..73314	-	561	WP_000139328.1	fertility inhibition protein FinO	-
LDB14_RS22755 (73369)	73369..74115	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LDB14_RS22760 (74135)	74135..74335	-	201	WP_072354025.1	hypothetical protein	-
LDB14_RS22765 (74360)	74360..75064	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LDB14_RS22770 (75119)	75119..75484	-	366	Protein_88	type IV conjugative transfer system protein TraE	-
LDB14_RS22775 (75504)	75504..75809	-	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaSHV-187 / blaCTX-M-65	-	1..138128	138128
	-	flank	IS/Tn	-	-	74360..75064	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T218226 WP_001312851.1 NZ_CP084206:c70738-70589 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T218226 NZ_CP115701:c4660649-4660116 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT218226 NZ_CP084206:70783-70842 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin